畜牧兽医学报2011，Vol.42Issue(12)：1655-1660,6.
瘤胃微生物植酸酶的分类与酶学性质研究进展
Research Advance in Classification and Enzymological Characteristics of Microbial Phytase in the Rumen
摘要
Abstract
Ruminants have the ability to utilize phytate phosphate by rumen microorganisms. Until now, domestic research on ruminal microbe phytase is insufficient. In this paper, phytase origin and activity in the rumen was briefly reviewed, and five kinds of rumen bacteria were capable of producing phytase. Among these bacteria, phytase activity was particularly prevalent in Sele-nomonas ruminantium (199. 1-703. 6 U ? mL-1). Based on their optimal pH and catalytic mechanisms, ruminal phytases are classified into acid phytase and cysteine phosphatases phytase, respectively. According to the stereospecificity of phytase, rumen microbes may exist 3-phytase and 5-phytase. Furthermore, kinetics of enzymatic reaction characteristics of phytases were summarized and discussed comprehensively. In a brief, phytase of S. Ruminantium has a optimal temperature of 50-55 'C , an optimal pH range of 4. 0-5. 5, and exhibits the highest specificity constant for wryo-inositol hexakisphosphate and ATP. The optimal phytase activity of Megasphaera elsdenii is displayed at pH5. 0 and 60 °C , and it also displays strict specificity for mjyo-inositol hexakisphosphate.关键词
瘤胃微生物/植酸酶/酶学特性Key words
rumen microorganism/ phytase/ enzymological characteristics分类
农业科技引用本文复制引用
沈莎莎,杨红建,张晓明..瘤胃微生物植酸酶的分类与酶学性质研究进展[J].畜牧兽医学报,2011,42(12):1655-1660,6.基金项目
国家现代奶牛产业技术体系(Nycytx-0402) （Nycytx-0402）
国家重点基础科学研究计划(2011CB100800) （2011CB100800）
