畜牧兽医学报2011，Vol.42Issue(12)：1696-1703,8.
运用多座位外显方差法分析中国荷斯坦牛CXCR1和IL8基因与乳房炎易感性的关系
Using the MPVA Model to Analyze the Impact of Gene-Gene Interaction between CXCR1 and IL8 2789/2862 on the Risk of Mastitis Susceptibility of Chinese Holstein
摘要
Abstract
The aims of this study were to identify the interaction of CXCRl and JL8 and their impacts on the risk of mastitis susceptibility of Chinese Holstein, and to test the feasibility of using Multi-Locus penetrance variance analysis (MPVA) model in analyzing gene-gene interactions. The polymorphisms of 7 SNP sites including the 5' flanking region and coding region of CXCRl and intron 3, exon 4 and exon 5 of IL8 were detected by PCR-SSCP and direct sequencing for 634 Chinese Holstein, including 102 individual with mastitic, from a northern farm of China, and to identify the interaction of CXCRl and IL8 and their impacts on the risk of mastitis susceptibility using MPVA. The interactions of CXCR1-1830-1768-IL8 2789/2862 showed significant effects on the mastitis susceptibility of cow using MPVA model. The results indicate that the interactions of CXCRM830-1768-IL8 2789/2862 is the best multi-genotype model to estimate the risk of mastitis susceptibility of Chinese Holstein. MPVA can be used to estimate the risk of mastitis susceptibility of Chinese Holstein.关键词
MPVA/CXCR1/IL8/乳房炎易感性Key words
MPVA/ CXCRl /IL8/ mastitis susceptibility分类
农业科技引用本文复制引用
张亚琴,杨章平,毛永江,陈仁金,陈莹,冀德君..运用多座位外显方差法分析中国荷斯坦牛CXCR1和IL8基因与乳房炎易感性的关系[J].畜牧兽医学报,2011,42(12):1696-1703,8.基金项目
国家高新技术研究发展计划(863)项目(2008AA10Z144) （863）
江苏省高校自然科学研究重点项目(09KJA230002) （09KJA230002）
江苏省高校自然科学基础研究项目(08KJB230005) （08KJB230005）
