畜牧兽医学报2011，Vol.42Issue(12)：1704-1711,8.
组织蛋白酶对成年绵羊卵母细胞体外发育能力的影响
The Effect of Cathepsin on in vitro Developmental Capacity of Oocytes from Adult Sheep
摘要
Abstract
This study was conducted to explore the mRNA expression of cathepsin (CTSB, CTSS) in cumulus cells during in vitro maturation(IVM) from sheep and to detect the correlation beween the mRNA expression of CTSB and CTSS in cumulus cells and in vitro developmental capacity of oocytes. The expression abundance of CTSB and CTSS in cumulus cells of sheep was detected at IVM 0 h,IVM 8 h,IVM 16 h and IVM 24 h through Real-time PCR. The expression abundance of CTSB and CTSS in cumulus cells was detected before and after IVM in the maturemedium which contains cathepsin inhibitor E-64 with different concentration. The effect of E-64 with different concentration in mature medium on in vitro maturation, fertilization and developmental capacity of oocytes were also observed. The mRNA expression of CTSB and CTSS in cumulus cells was down-regulated continuously during in vitro maturation (IVM 0 h, IVM 8 h and IVM 16 h, P<0. 01), the mRNA expression of CTSB and CTSS in the groups with 5 and 10 μmol ? L-1 E-64 for IVM 24 h were significantly lower than that of the control and 1 μmol ? L-1 E-64 groups, nevertheless, there were no significant difference between the two former groups (P>0. 05). The blastocysts rate in the groups of 5 and 10 μmol ? L-1 E-64(26. 82%, 30. 95%) were higher than those of the control and 1 μmol ? L-1 E-64 groups(15. 63%, 18. 23%, P< 0. 05), but there were no significant difference in the percentage of oocytes mature and cleavage rate among all the groups (P>0. 05). The result demonstrate that the mRNA expression abundance of CTSB and CTSS in cumulus cells from sheep is down-regulated continuously during IVM, there is negative correlation between the mRNA expression of CTSB and CTSS in cumulus cells and the following embryo developmental capacity;the cathepsin inhibitor E-64 could improve the capacity of oocytes developing into blastocysts after fertilization by down-regulating the mRNA expression abundance of CTSB and CTSS in cumulus cells.关键词
E-64/卵丘细胞/组织蛋白酶/体外发育/Real-time PCRKey words
E-64/ cumulus cells/ cathepsin/ development in vitro /Real-time PCR分类
农业科技引用本文复制引用
刘志涛,田树军,孙树春,陈晓勇,张艳普,胡媛媛,郭军艾..组织蛋白酶对成年绵羊卵母细胞体外发育能力的影响[J].畜牧兽医学报,2011,42(12):1704-1711,8.基金项目
国家科技支撑计划(2011BAD191302) （2011BAD191302）
教育部科学技术研究重点项目(211018) （211018）
