西南国防医药2012，Vol.22Issue(1)：22-24,3.DOI:10.3969/j.issn.1004-0188.2012.01.007
基于CO1基因序列的DNA条形码在中尼边境鼠类物种鉴定中的应用
Application of DNA bar code based on CO1 gene order in identification of murid species in Sino-Nepali border area
陈春生 1潘其礼 2王静 2张晓龙 2张雪莲 3范泉水 3牛文忠 3胡小兵 3格龙 4房键慧 2曹晓梅2
作者信息
- 1. 850002,拉萨,西藏出入境检验检疫局
- 2. 中国检验检疫科学研究院
- 3. 成都军区疾病预防控制中心
- 4. 西藏地方病防治所
- 折叠
摘要
Abstract
Objective To discuss the feasibility of using mitochondrial CO1 gene as DNA bar code to identify species of murid. Methods The genome DNA was extracted from 42 murid samples collected in the Sino - Nepali border area. The mitochondrial C01 gene was amplified by method of consensus primer PCR and sequenced. BLAST comparison was made between the sequencing results and the DNA bar codes of other murid species in genebank,and molecular evolution tree was built. Results In this research, specific C01 gene straps in 17 samples were amplified by PCR. The average intraspecies genetic distance of the 17 samples was 0.035. The average interspecies genetic distance was 0. 47. The interspecies genetic distance was almost 10 times more than the intraspecies genetic distance. According to the BLAST comparison, the 17 samples were classified in different murid species. Conclusion The C01 gene order can be used effectively to identify the murid species.关键词
鼠类分类/CO1基因/DNA条形码/分子进化树Key words
classification of murid/CO1 gene/DNA bar code/molecular evolution tree分类
生物科学引用本文复制引用
陈春生,潘其礼,王静,张晓龙,张雪莲,范泉水,牛文忠,胡小兵,格龙,房键慧,曹晓梅..基于CO1基因序列的DNA条形码在中尼边境鼠类物种鉴定中的应用[J].西南国防医药,2012,22(1):22-24,3.
