纤维素科学与技术2011，Vol.19Issue(4)：6-13,8.
金属离子对纤维素酶内切酶和外切酶活性的影响
Influence of the Metal Ions on the Activity of Endo-1,4-β-D-glucanase and Exo-1,4-β-D-glucanase
张洪鑫 1陈小泉 1蒋玲玲1
作者信息
- 1. 华南理工大学制浆造纸工程国家重点实验室,广东广州510640
- 折叠
摘要
Abstract
Because of the metal ions can affect the activity of endo-l,4-13-D-glucanase （EG） and exo-1,4-β-D-glucanase （CBH） on cellulose and different metal ions have the different activity. Accordingly, we select three kinds of cellulase and do the single-factor experiment. The activity of EG and CBH on cellulase were results showed： The activity of EG can improve effectively when the concentration of K＋ is 0.9 mg/mL, Ca2＋ 0.3 mg/mL, Zn2＋ 1.2 mg/mL, Cu2＋ 1.2 mg/mL; Mg2＋, Ca2＋, Ba2＋, Cu2＋, Zn2＋ showed the inhibitory character. The Al3＋ had some inhibition on the cellulase activity of EG and CBH. Anions with different valences have no effect on the activity of EG and CBH.关键词
纤维素酶/内切酶/外切酶/酶活：金属离子Key words
cellulase/endo-1,4-β-D-glucanase/exo-1,4-β-D-glucanase/enzyme activity/metal ion分类
生物科学引用本文复制引用
张洪鑫,陈小泉,蒋玲玲..金属离子对纤维素酶内切酶和外切酶活性的影响[J].纤维素科学与技术,2011,19(4):6-13,8.
