云南农业大学学报2011，Vol.26Issue(6)：776-780,5.DOI:10.3969/j.issn.1004-390X(n).2011.06.008
乌金猪基因组文库的构建
Construction of Genomic Library of Wujin Pig
摘要
Abstract
To identify the genomic mechanisms for specific traits of Wujin pig, a domestic Chinese swine breed, we constructed its genomic library. The genomic DNA larger than 50 kb was extracted from Wujin pig liver tissue and was digested randomly by restriction enzyme Bel I. DNA fragments ranging from 10 to 23 kb were recovered by agarose gel electrophoresis. EMBL3 vector was digested by BamH I, treated with calf intestine alkaline phosphatase, and then liga-ted with purified DNA fragments forementioned. The genomic library of Wujin pig was constructed by packing recombinant DNA in vitro with phage package protein and transecting KW251 host cells. The result showed that the titer of the library was 2. 4 × 109 pfu/mL. The genomic library of Wujin pig could be used for identification of genes and genomic regions of interest, and provide valuable data for further study such as cloning of genomic DNA and functional analysis of regulatory element.关键词
乌金猪/基因组文库/基因克隆Key words
Wujin pig/ genomic library/ gene clone分类
农业科技引用本文复制引用
张永云,李卫真,赵素梅,黄英,高士争..乌金猪基因组文库的构建[J].云南农业大学学报,2011,26(6):776-780,5.基金项目
国家"973"前期研究专项(2007LS700101). （2007LS700101）
