重庆高教研究2011，Vol.30Issue(5)：34-37,4.
基于聚硫堇/纳米金固定酶的过氧化氢生物传感器
Hydrogen peroxide biosensor based on immobilized Enzyme of poly-thionine/ gold Nanoparticles
摘要
Abstract
A new approach to measure hydrogen peroxide（H2O2） with HRP/nano-Au/ PTH /GC modified electrode has been developed.First,a stable poly-thione film was polymerization on glassy carbon electrode.Then nano-gold and horseradish peroxidase was successful immobilized onto the above prepared film to construct the hydrogen peroxide biosensor via the effect of electrostatic adsorption and process of covalent bonding.The performance of the biosensor was characterized by cyclicvoltammetry and the chronoamperometry.The results confirmed that the electrode exhibited high sensitivity,fast response,and linear response in the range of 1.4×10-6 molL~4.26×10-3 molL with correlation coefficients of 0.9993（n=23）,respectively,and the detection limit of 4.0×10-7 molL（SN=3）.关键词
聚硫堇/纳米金/过氧化物酶/生物传感器Key words
poly-thionine/nano-gold/Horseradish peroxidase（HRP）/biosensor分类
信息技术与安全科学引用本文复制引用
黄小梅,邓祥,吴狄..基于聚硫堇/纳米金固定酶的过氧化氢生物传感器[J].重庆高教研究,2011,30(5):34-37,4.基金项目
四川文理学院科研资助项目 （）
