摘要
Abstract
Objective To investigate protective effects of ligustrazine ( tetramethypyrazine, TMP) on cisplatin-induced oxidative injury of kidneys in rats. Methods TMP was administered at 50,100 mg ·kg-1· d body weight intraperitoneally (ip) for five consecutive days, 2 days before a single dose of cisplatin (DDP, 8 mg · kg-1 ,ip). The rats were sacrificed four hours after the last injection. Blood urea nitrogen( BUN) and serum creatinine (SCr) were tested, 24 hours urine were collected to detect urinary protein. The contents of malondialdehyde (MDA) , glutathione (GSH) content, nitric oxide (NO) , activities of superoxide dismutase ( SOD) , glutathione-S-transferase ( GST) , the nitric oxide synthase ( NOS) in the renal cortex were measured. Results Compared with DDP, TMP (50 and 100 mg ·kg-1· d-1 ) dose-dependently reduced 24 h urinary protein, BUN and SCr induced by cisplatin, by 41.45% ,65. 33% ;18. 12% ,57. 51% ;14. 39% ,48. 89% (P<0. 05 , P<0. 01) , respectively. The 100 mg · kg-1· d-1 TMP significantly decreased levels of MDA, NO, NOS and GSH, SOD, GST induced by cisplatin in kidney by 36. 73% , 45. 39% , 22. 86% ( P<0. 05) , respectively; while, the content of GSH was increased by 1. 33-fold, 1.66-fold ,1.57-fold (P<0. 05), respectively, compared with that in the DDP group. Conclusion TMP could protect cisplatin-induced oxidative nephrotoxicity.关键词
川芎嗪/顺铂/氧化损伤/保护作用Key words
Ligustrazine/Cisplatin/Oxidative damage/ Protection分类
医药卫生
