医药导报2012，Vol.31Issue(2)：156-159,4.DOI:10.3870/yydb.2012.02.007
二苯乙烯苷对人脐静脉内皮细胞一氧化氮合酶及其基因的调节作用
Effects of 2,3,5 4'-tetrahydroxystilbene-2-O-β-D-glucoside on Nitric Oxide Synthase and Gene Expression in Endothelial Cells of Human Umbilical Vein
沈建芬 1张又枝 2肖军花 2王嘉陵2
作者信息
- 1. 浙江省嘉兴市第一医院,314000
- 2. 华中科技大学同济医学院基础医学院药理系,武汉,430030
- 折叠
摘要
Abstract
Objective To investigate the effects of 2, 3, 5 4'-tetrahydroxystilbene-2-O-β-D-glucoside ( THSG ) on content of nitric oxide (NO) and activity of endothelial nitric oxide synthase (eNOS) and the mechanisms. Methods The human umbilical vein endothelial cells ( HUVECs ) were cultured and divided into four groups: the control, THSG ( 1, 10, 100 μmol·L-1) groups. Spectrophotometry endothelium assay was used to measure NO and activity of eNOS. RT-PCR was used to analyze the eNOS mRNA expression and Western Blot was used to analyze eNOS protein expression level in HUVECs. Results THSG significantly increased the production of NO, activated NOS and up-regulated expression of eNOS mRNA and protein in a concentration-dependent manner compared with the control group. Conclusion THSG raises expression of eNOS mRNA and protein, which may result in activating NO production and relaxing blood vessels.关键词
二苯乙烯苷/人脐静脉内皮细胞/一氧化氮/一氧化氮合酶Key words
2,3,5 4'-tetrahydroxystilbene-2-O-β-D-glucoside/Human umbilical vein endothelial cells/ Nitric oxide/ Endothelial nitric oxide synthase分类
医药卫生引用本文复制引用
沈建芬,张又枝,肖军花,王嘉陵..二苯乙烯苷对人脐静脉内皮细胞一氧化氮合酶及其基因的调节作用[J].医药导报,2012,31(2):156-159,4.
