医药导报2012，Vol.31Issue(2)：202-205,4.DOI:10.3870/yydb.2012.02.028
柱前衍生化法测定晶帽石斛中总氨基酸含量
Contents of Total Amino Acids in Dendrobium crystallinum Tchb.F.with Pre-column Derivatization Method
摘要
Abstract
Objective To analyze the contents of amino acids in Dendrobium crystallinum Tchb. F. by using pre-column derivatization method. Methods The amino acids in Dendrobium devonianum were pretreated with 2, 4-dinitro-fluorobenzene and their derivatives were analyzed on Waters 600 HPLC using Symmetry C,8 column. Gradient eluent consisted of sodium acetate solution (pH = 6.4) with 1% N,N-dimethylfonnamide, acetonitrile and water (1:1) was taken. The detection wavelength was 360 nm and column temperature was 35 ℃ and loading volume was 20 μL. Results The calibration curves for amino acids showed good linearity ( r = 0. 999 5 - 1.000 0,re = 5). The mean recoveries of amino acids were 99. 34% -101.93% with RSD as 0. 54% -1. 91% (re = 6). And the average content of amino acids was 3. 79%. Conclusion The method is quick, simple with minimum loss of amino acids, which provides a theoretical basis for the Dendrobium crystallinum development.关键词
晶帽石斛/氨基酸/柱前衍生化/色谱法/高效液相Key words
Dendrobium crystallinum Tchb. F./Aamino acids/ Pre-column derivatization/ HPLC分类
医药卫生引用本文复制引用
李光,李学兰,陈曦..柱前衍生化法测定晶帽石斛中总氨基酸含量[J].医药导报,2012,31(2):202-205,4.基金项目
云南省科技重大项目"傣药南药特色资源评分及保育技术研究"(2008IF018) （2008IF018）
中国医学科学院药用植物研究所中央级公益性科研院所基本科研业务专项(YZYN-10-01) （YZYN-10-01）
