医药导报2012，Vol.31Issue(3)：282-285,4.DOI:10.3870/yydb.2012.03.004
精制蛇毒酶的毒理学研究
Toxicological Study of Purified Snake Venom Enzyme
摘要
Abstract
Objective To evaluate the safety of purified snake venom enzyme ( PSE ). Methods One hundred mice were randomly divided into two groups for the median lethal dose ( ivLDjQ and ipLDjQ ) test through tail vein injection and in-traperitoneal injection, respectively. A total of 120 mice and 40 rabbits were randomly divided into four groups for the effects stud-y of the tested groups at different doses. The mice were administered PSE for 90 days continuously and rabbits were treated with PSE for 45 days ( every other day ). On the 91st day,blood was collected and ALT,BUN and routine blood testing were carried out. The liver and kidney tissues were moved for pathological examination. Results The ivLD50 of PSE was 6. 695 U · kg-1 , 90% confidence interval was 5. 434-7. 956 U · Kg-1 ; ip LD50 of PSE was 4. 599 U · Kg-1 ,90% confidence interval was 3. 735-5.462 U · kg-1 . Compared with that of the control group, no significant difference ( P>0. 05 ) was showed on the function of liver and kidney and blood indicators. Conclusion PSE has low toxicity at the experimental doses. Since the amount of enzyme is 1. 25x10-2 U · g-1 in cream,the dosage for adults ( 0.05 g · cm-1 equivalent to 6. 25x10-4 U · cm-1 enzyme activity ) is safe and reliable.关键词
蛇毒酶,精制/毒性,急性/毒性,慢性/半数致死量Key words
Snake venom enzyme, purified ( PSE )/ Toxicity, acute/ Toxicity, chronic/ LD50分类
医药卫生引用本文复制引用
刘海军,梁再赋,詹丽芬,孙晋民..精制蛇毒酶的毒理学研究[J].医药导报,2012,31(3):282-285,4.基金项目
辽宁省科学技术计划基金资助项目(2008225021-1) （2008225021-1）
