医药导报2012，Vol.31Issue(4)：495-497,3.DOI:10.3870/yydb.2012.04.031
青风藤中总生物碱的气相色谱-质谱联用分析
Analysis of Total Alkaloids in Sinomenium acutum by Gas Chromatography-Mass Spectrometry
吴振启 1郭剑明 2陈琴华 3李慧峰 1李伟亮 1张菊根 1王国民2
作者信息
- 1. 复旦大学附属中山医院青浦分院泌尿外科,上海,201700
- 2. 复旦大学中山医院泌尿外科,上海200032
- 3. 武汉大学药学院,430070
- 折叠
摘要
Abstract
Objective To extract total alkaloids of Sinomenium acutum by Soxhlet extractor and analyze them by gas chromatography-mass spectrometry (GC-MS). Methods The Sinomenium acutum was extracted by 90% alcohol, acidified by hydrochloric acid, alkalinized by ammonia water, and then extracted by chloroform. Fifteen peaks were separated and identified by GC-MS. Results The relative content of sinomenine, ambelline and stepharine was up to 80. 18% , 3.44% and 3.05% , respectively. The compounds were quantitatively determined by normalization method, and the identified compounds represented 95. 1% of total GC peak area. Conclusion The results provide important foundation to further study and exploit Sinomenium acutum.关键词
青风藤/青藤碱/总生物碱/气相色谱-质谱联用Key words
Sinomenium acutum/ Sinomenine/ Total alkaloids/ Gas chromatography-mass spectrometry分类
医药卫生引用本文复制引用
吴振启,郭剑明,陈琴华,李慧峰,李伟亮,张菊根,王国民..青风藤中总生物碱的气相色谱-质谱联用分析[J].医药导报,2012,31(4):495-497,3.
