应用化学2011，Vol.28Issue(12)：1429-1435,7.DOI:10.3724/SP.J.1095.2011.00766
乙酰胆碱酯酶/Nafion/普鲁士蓝修饰玻碳电极的制备及其在有机农药检测中的应用
Preparation of Acetylcholinesterase/Nafion/Prussian Blue Modified Glassy Carbon Electrode and Its Application in Pesticide Detection
摘要
Abstract
This paper reports the preparation of an acetylcholinesterase ( AChE) /Nafion/Prussian blue(PB)/ glass carbon electrode (GCE) and the electrochemical detection of pesticides such as carbaryl and trichlorfon. Atomic force microscopy and electrochemical technique were used to investigate the construction of the modified electrode and its effects on pesticide detection. Those results indicated that AChE dispersed uniformly in Nafion/PB/GCE. The AChE/Nafion/PB/GCE constructed under optimal conditions showed a good linear relationship between the peak current and the concentration of pesticide in the range of 0. 01 ~0. 5 (junol/L & 2. 0 ~ 10. 0 u,mol/L for carbaryl and 0. 02 ~ 1. 0 ujnol/L & 2. 0 ~ 8. 0 jjimol/L for trichlorfon, respectively. The limit of detection is 5. 0 nmol/L for carbaryl and 10. 0 nmol/L for trichlorfon, respectively. Furthermore the modified electrode was applied in the detection of analogous practical samples. It was found that the as-prepared sensor had high detection sensitivity, good reproducibility and anti-interference.关键词
西维因/敌百虫/普鲁士蓝/Nafion/乙酰胆碱酯酶/电化学Key words
carbaryl/ trichlorfon/ prussian blue/ Nafion/ acetylcholinesterase/ electrochemistry分类
化学化工引用本文复制引用
张梅,汪莉,袁慧珍,刘丽,宋永海,叶树虹,肖贤平,王菁苒,刘慧萍..乙酰胆碱酯酶/Nafion/普鲁士蓝修饰玻碳电极的制备及其在有机农药检测中的应用[J].应用化学,2011,28(12):1429-1435,7.基金项目
国家自然科学基金(20905032,21065005)、江西省自然科学基金(2008GZH0028)、江西省教育厅科技项目(GJJ10389)、江西师范大学青年成长基金、江西师范大学科研启动基金和江西师范大学研究生创新基金(YJS2010060)资助项目 （20905032,21065005）
