应用生态学报2011，Vol.22Issue(12)：3259-3264,6.
石油污染地土壤微生物群落的碳源利用特性
Carbon source utilization characteristics of microbial communities in a petroleum-contaminated soil in Daqing Oil Field, Northeast China
摘要
Abstract
By using Biolog technique, this paper studied the carbon source utilization characteristics of microbial communities in different layers (0-10 cm, 10-20 cm, and 20-30 cm) of a petroleumcontaminated soil near an oil well having exploited for 36 years in Daqing Oil Field. Petroleum contamination enhanced the metabolic activity of the soil microbial communities obviously. In the three layers of the petroleum-contaminated soil, the metabolic activity of the microbes was higher than that of the control, and there existed significant differences between different layers of the petroleum-contaminated soil. The carbon source metabolic capacity of the microbes in different layers of the petroleum-contaminated soil was in the order of 20-30 cm >10-20 cm > 0-10 cm. Petroleum contamination made the kinds of soil carbon source and the metabolic diversity of soil microbes increased, being more obvious in 10-20 cm and 20-30 cm soil layers but less change in 0-10 cm soil layer. In the contaminated soil, the majority of the carbon sources utilized by the microbes in 10-20 cm soil layer were carbohydrates instead of the carboxylic acids in non-contaminated soil, whereas the majority of the carbon substrates utilized in 20-30 cm soil layer were carboxylic acids. All the results suggested that petroleum-contaminated soil had its unique microbial community structure and peculiar microbial carbon source utilization characteristics.关键词
石油污染/土壤微生物群落/Biolog/碳源利用Key words
petroleum contamination/ soil microbial community/ Biolog/ carbon source utilization.分类
能源科技引用本文复制引用
岳冰冰,李鑫,任芳菲,孟凡娟,尹鹏达,张会慧,孙广玉..石油污染地土壤微生物群落的碳源利用特性[J].应用生态学报,2011,22(12):3259-3264,6.基金项目
国家自然科学基金项目(30771 746,31070307)和黑龙江省重点科技攻关项目(GA07B306)资助. （30771 746,31070307）
