应用生态学报2011，Vol.22Issue(12)：3300-3306,7.
粗毛栓菌漆酶的诱导及其对中性染料和有机磷农药的降解
Induce of laccase from Trametes gallica and its degradation on neutral dyes and organophosphorus pesticides
摘要
Abstract
The characteristics of the induction of laccase in Trametes gallica under different initial cultural pH, incubation time by different inducers were discussed, as well as the effects of temperature , pH and time on laccase degradation of six dyes and four organophosphors. The results showed that RB-bright blue, ABTS and o-toluidine affected the production of laccase at different levels, and ABTS was the best inductive agent in our test conditions, whose optimal initial pH and incubation time were 4.0 and 13 days, respectively. The appropriate reaction temperature of the laccase produced was 38 °C , and it got a good stability, for it could retain 78.6% of the enzyme activity after 20 min holding at 40 °C. Mediated by ABTS, the optimal temperature for laccase to degrade the six types of neutral dyes could be divided into two cases, that was 30℃ ( neutral black, neutral bordeaux, neutral pink, methyl orange) and 60℃(neutral dark yellow, cresol red) , the optimal pH were 6.0 (neutral black) , 2.0 (neutral bordeaux, neutral pink) and 4.0 (methyl orange, neutral dark yellow, cresol red) , respectively, while the optimal times separately were 6 h (methyl orange, neutral dark yellow, cresol red), 12 h (neutral pink) and 24 h (neutral bordeaux). And using the same inductive agent, the best temperature for laccase to degrade dimethoate, chlorpyrifos, trichlorfon and parathion-pyridazine was 25 X., the suitable time was 9 h, and the optimal pH was 10.0 for dimethoate, chlorpyrifos and parathion-pyridazine, and 8.0 for trichlorfon.关键词
粗毛栓菌/漆酶/难降解物/降解作用Key words
Trametes gallica/ laccase/ biorefractory compound/ degradation分类
资源环境引用本文复制引用
靖德军,黄剑波,杨洲平,胡容,程子彰,黄乾明..粗毛栓菌漆酶的诱导及其对中性染料和有机磷农药的降解[J].应用生态学报,2011,22(12):3300-3306,7.基金项目
四川省教育厅重点科研项目(08ZA053)和四川农业大学科技创新基金项目(00731200)资助. （08ZA053）
