针刺研究2011，Vol.36Issue(6)：409-413,5.
眼针对脑缺血再灌注损伤大鼠脑组织细胞间黏附因子-1表达的影响
Effect of Eye-acupuncture on Cerebral Intracellular Adhesion Molecule-1 expression in Rats with Acute Cerebral Ischemia-reperfusion Injury
摘要
Abstract
Objective To investigate the effect of the eye-acupuncture therapy on cerebral intercellular adhesion molecule-1 (ICAM-1) expression in rats with acute cerebral ischemia-reperfusion injury (CI/RI) so as to reveal its underlying mechanism. Methods Forty male SD rats were randomly and equally divided into normal, sham operation (sham), model and eye-acupuncture groups. Acute CI/RI model was established by occlusion of the middle cerebral artery with a modified suture-blocking method. Filiform needles were inserted into the "Qan" (Liver Area), "Shangjiao" (Upper Energizer Area), "Xiajiao" (Lower En-ergizer Area), etc. Of the eye-acupuncture acupoints, and retained for 20 min. The treatment was conducted twice daily, 7 times altogether. Seventy-two hours after Acute CI/RI, the neurological behavior was assessed by ZeaLonga neurophysical impairment scale. Cerebral ICAM-1 protein and ICAM-1 mRNA expression levels were detected by Western blot (Penumbra area of the right cerebral infarction region) and real time-polymerase chain reaction (PCR) methods, respectively. Results After modeling, the neurophysical impairment score of the model group was increased from 0 to (2. 63 ± 0. 92) points on the first day and (2. 63 ± 0. 74) points 72 h following Acute CI/RI. Correspondingly, cerebral ICAM-1 protein and mRNA expression was up-regulated considerably in the model group than in the normal control group (P<0. 01). In comparison with the model group, the neurologic impairment score, and cerebral ICAM-1 protein and mRNA expression levels of the eye-acupuncture group were down-regulated obviously ( P<0. 01, P<0. 05). No significant differences were found between the normal control and sham groups in neurologic impairment score and the expression levels of the ICAM-1 protein and ICAM-1 mRNA(P>0.05). Conclusion Eye-acupuncture therapy can improve the cerebral ischemia-reperfusion injury induced behavior changes, which may be related to its effects in down-regulating the expression of cerebral ICAM-1 protein and gene.关键词
眼针/脑缺血再灌注损伤/细胞间黏附因子/脑组织Key words
Eye-acupuncture/ Cerebral ischemia-reperfusion injury/ intercellular adhesion molecule/ Cerebral tissue分类
医药卫生引用本文复制引用
张立德,于丹,曲怡,王哲,高原,潘茜,王健,关洪全..眼针对脑缺血再灌注损伤大鼠脑组织细胞间黏附因子-1表达的影响[J].针刺研究,2011,36(6):409-413,5.基金项目
国家重点基础研究发展计划("973"计划)项目(2007 CB 512702)、辽宁省教育厅课题(L 2010351) （"973"计划）
