诊断学理论与实践2011，Vol.10Issue(6)：548-552,5.
自动化DiversiLab System DNA指纹分型系统在白假丝酵母同源性分析中的应用
Application of automated DiversiLab System DNA fingerprinting in homologous analysis of Candida albicans
摘要
Abstract
Objective To apply automated DiversiLab System DNA fingerprinting in homologous analysis of Candida alb icons and evaluate its performance. Methods PCR amplification was performed using standardized reagent kits and its product was detected by microfluidics-based electrophoresis and Agilent 2100 Bioanalyzer. The DNA fingerprinting data was interpreted by Web-based software. Results Among the 35 strains of Candida alb icons isolated, 7 types were classified, including A type(13 strains), B type(8 strains), C type(6 strains), D type(3 strains), E type (2 strains), F type (2 strains) and G type (1 strain). Two isolates with fluconaozle-resistance belonged to B type , and four isolates of fluconazole-susceptible-dose-dependent belonged to C type. Conclusions Automated DiversiLab System offers efficient and excellent discriminatory power with high reproducibility and allows the building of database and inter-laboratory comparisons. It could be recommended as a first-line tool in the identification and typing of Candida alb wans.关键词
DiversiLab系统/白假丝酵母/重复序列聚合酶链反应/同源性分析Key words
DiversiLab system/ Candida albicans/ Repetitiveelement-based polymerase chainreaction/Homologous analysis分类
医药卫生引用本文复制引用
刘锦燕,项明洁,倪培华,张华,陈华,倪语星..自动化DiversiLab System DNA指纹分型系统在白假丝酵母同源性分析中的应用[J].诊断学理论与实践,2011,10(6):548-552,5.基金项目
上海市卫生局课题(2009239) （2009239）
上海交通大学医学院课题(09XJ21036) （09XJ21036）
