中国病理生理杂志2012，Vol.28Issue(2)：216-221,6.DOI:10.3969/j.issn.1000-4718.2012.02.005
人Gax基因转染对血管平滑肌细胞增殖、迁移和细胞周期的影响
Effects of human growth arrest-specific homeobox gene delivery on proliferation, migration and cell cycle distribution of vascular smooth muscle cells
摘要
Abstract
ADM: To explore a new gene therapeutic strategy for vein graft restenosis by investigating the effects of adenovirus - mediated human growth arrest - specific homeobox ( Ad5 - hGax ) gene delivery on the proliferation, migration and cell cycle distribution of serum - induced rabbit vascular smooth muscle cells ( VSMCs ). METHODS: The recombinant adenovirus vector containing hGax gene was constructed and transfected into rabbit VSMCs. The expression of hGax in VSMCs was detected by RT - PCR and immunofluorescent staining. Methyl thiazolyl tetrazolium ( MTT ) assay was used to assess the effect of hGax over - expression on serum - induced proliferation of VSMCs. Wound healing method was applied to examine the distance of serum - induce VSMCs migration. Flow cytometry was employed to analyze the cell cycle distribution. RESULTS: The recombinant adenovirus vector Ad5 - hGax was successfully constructed. The results of RT - PCR and immunofluorescent staining revealed that the hGax - transfected cells contained a 174 bp specific fragment of hGax gene and target protein 48 h after transfection. The proliferation of serum - induced VSMCs was significantly inhibited by overexpression of hGax gene as compared with control group. The migration of serum - induced VSMCs was inhibited after hGax gene delivery. Flow cytometry showed that 72 h after serum induction, the cells in G0/G1 phase in Ad5 - hGax group were significantly increased, whereas the cells in G2/M + S phase were significantly decreased. CONCLUSION: Overexpression of hGax gene inhibits the proliferation and migration of serum - induced rabbit VSMCs, and arrests the cells in G0/G1 phase. It is likely that hGax gene is a potential target for the gene therapy of vein graft restenosis.关键词
同源盒/转染/血管平滑肌细胞/细胞增殖/细胞运动/细胞周期Key words
Homeobox/ Transfection/ Vascular smooth muscle cells/ Cell proliferation/ Cell movement/ Cell cycle分类
医药卫生引用本文复制引用
郑辉,薛松,连锋,胡振雷,汪永义..人Gax基因转染对血管平滑肌细胞增殖、迁移和细胞周期的影响[J].中国病理生理杂志,2012,28(2):216-221,6.基金项目
上海市科委基金资助项目(No.08411964100) （No.08411964100）
