中国病理生理杂志2012，Vol.28Issue(2)：222-227,6.DOI:10.3969/j.issn.1000-4718.2012.02.006
过氧化物酶体增殖物激活受体δ对同型半胱氨酸诱导人脐静脉内皮细胞MCP-1mRNA表达的影响及机制
Effect of peroxisome proliferator activated receptor δ on mRNA expression of MCP-1 induced by homocysteine in cultured human umbilical vein endothelial cells
摘要
Abstract
AIM: To investigate the effects of peroxisome proliferator activated receptor 8 ( PPAR8 ) on the mRNA expression of monocyte chemoattractant protein 1 ( MCP - 1 ) induced by homocysteine ( Hey ) in human umbilical vein endothelial cells ( HUVECs ). METHODS: Collagenase was used to isolate endothelial cells from human umbilical vein, and the cells were cultured in vitro. The HUVECs were divided into blank control group, Hey group, GW0742 ( a specific agonist of PPAR8 ) group and diphenyleneiodonium ( DPI, a specific inhibitor of NADPH oxidase ) group. RT -PCR was used to examine the mRNA expression of MCP - 1 and PPAR8. The protein level of PPAR8 was detected by Western blotting. 2',7' - Dichlorofluorescin diacetate( DCFH - DA ) was added to monitor intracellular production of reactive oxygen species ( ROS ). RESULTS: Compared with control group, Hey promoted the mRNA expression of MCP - 1 in a concentration - dependent manner, and decreased the mRNA expression of PPAR8 in HUVECs. The mRNA expression of MCP - 1 was significantly elevated by Hey at the concentration of 10 -5 mol/L, and the mRNA expression of PPAR8 was decreased remarkably ( P <0. 01 ). GW0742 decreased the mRNA expression of MCP - 1 compared with Hey group ( P < 0. 01 ). Hey remarkably increased the production of ROS compared with control group. Hey - induced production of ROS was also significantly attenuated by GW0742. CONCLUSION: The activation of PPAR8 decreases the Hey - induced mRNA expression of MCP - 1 by suppressing Hey - stimulated production of ROS.关键词
人脐静脉内皮细胞/同型半胱氨酸/过氧化物酶体增殖物激活受体δ/单核细胞趋化蛋白1Key words
Human umbilical vein endothelial cells/ Homocysteine/ Peroxisome proliferator activated receptor δ/ Monocyte chemoattractant protein 1分类
医药卫生引用本文复制引用
宋欢欢,徐尚华,黄茂芝,许昌声,谢良地..过氧化物酶体增殖物激活受体δ对同型半胱氨酸诱导人脐静脉内皮细胞MCP-1mRNA表达的影响及机制[J].中国病理生理杂志,2012,28(2):222-227,6.基金项目
福建省科技厅基金资助项目[No.(2007)3129] （2007）
