中国病理生理杂志2012，Vol.28Issue(2)：228-233,6.DOI:10.3969/j.issn.1000-4718.2012.02.007
高盐高脂摄入致大鼠主动脉重构及辛伐他汀的阻断作用
Retarding effect of simvastatin on artery remodeling induced by high-salt and high-fat diet in rats
摘要
Abstract
AIM: To investigate the effect of simvastatin intervention on the changes of blood pressure, serum lipid fluctuation and aortic configuration induced by high - sodium and high - fat diet in rats. METHODS: Sixty adult male SD rats were randomly divided into 5 groups ( n = 12 ): control ( N )group, high salt ( S )group, high fat ( F ) group, high salt + high fat ( SF ) group and high salt + high fat + simvastatin ( T ) group. After fed for 16 weeks, the rats were subject to determine blood pressures and serum concentrations of triglycerides ( TG ), total cholesterol( TC ) and soluble CD40 ligand ( sCD40L ). The expression of CD40/CD40L in the root of ascending aorta was detected by immunohistochem-ical method. The thickness of intima media in the ascending aorta as well as the ratio of lumen area/total vascular area were measured and calculated after HE staining. RESULTS: In S group, F group and SF group, systolic blood pressure wassignificantly higher than that in N group ( P <0.01 ). Systolic blood pressure in T group were slightly higher than that in N group with statistical significance and significantly lower than that in SF group. The serum concentrations of TG and TC in F group and SF group were significantly higher than those in N group and T group ( P < 0. 01 ), and no significant difference among S group, N group and T group was observed. In S group, F group and SF group, the serum concentrations of sCD40L were higher than that in N group and T group ( P < 0.05 ), meanwhile that in SF group was also higher than that in S group and F group ( P <0.05 ). However, no significant difference of sCD40L concentration between S group and F group as well as N group and T group was observed. The expression of CD40/CD40L in the ascending aorta in S group, F group and SF group was higher than that in N group and T group ( P <0. 05 ), and that in SF group was also higher than that in S group and F group ( P <0. 05 ). No significant difference of CD40/CD40L expression between S group and F group as well as N group and T group was observed. The thickness of intima media in S group, F group and SF group was significantly thicker than that in N group ( P <0. 01 ), and no significant difference of the intima media thickness between T group and N group was observed. The ratio of lumen area/total vascular area in S group, F group and SF group was smaller than that in N group ( P <0. 05 ), and no significant difference of the ratio between T group and N group was found. CONCLUSION : Feeding high - fat and high - salt diet leads to blood pressure elevation, induces atherosclerosis, increases serum concentration of sCD40L and enhances the expression of CD40/CD40L in arterial tissues. The combination of the stimuli has stronger effect than a single factor. Statins protect the arterial tissues against atherosclerosis by decreasing the level of serum sCD40L and inhibiting the arterial expression of CD40/CD40L.关键词
动脉粥样硬化/血管重构/辛伐他汀/CD40/CD40配体Key words
Atherosclerosis/ Vascular remodeling/ Simvastatin/ CD40/ CD40 ligand分类
医药卫生引用本文复制引用
尹明景,温汉春,叶永维,管庆林,黄皓章,黄露霜,朱继金..高盐高脂摄入致大鼠主动脉重构及辛伐他汀的阻断作用[J].中国病理生理杂志,2012,28(2):228-233,6.基金项目
广西自然科学基金资助项目(No.桂科自0833148) （No.桂科自0833148）
广西医疗卫生重点科研课题(No.重2010010) （No.重2010010）
