中国病理生理杂志2012，Vol.28Issue(2)：258-262,5.DOI:10.3969/j.issn.1000-4718.2012.02.013
聚腺苷二磷酸核糖糖苷水解酶调节癫痫大鼠海马组织凋亡诱导因子及炎症因子的研究
Poly(adenosine diphosphate ribose) glycohydrolase regulates apoptosis-inducing factor and inflammatory factors in rat hippocampus after seizures
摘要
Abstract
AIM: To investigate the effects and molecular mechanisms of poly( adenosine diphosphate ribose ) glycohydrolase ( PARG ) on rat hippocampus neurons after seizures and to study the effects of gallotannin on the expression of apoptosis - inducing factor ( AIF ), interleukin - 1 β ( IL - 1 β ) and tumor necrosis factor - a ( TNF - a ) in rat hippocampus after seizures. METHODS: Seizures were induced by kainic acid ( KA ). The damage of hippocampus neurons was evaluated by Nissl staining. The protein expression levels of poly( adenosine diphosphate ribose ) ( PAR ), AIF, IL -1 β and TNF - a were detected by Western blotting analysis. RESULTS: The number of damaged hippocampal pyramidal neurons in gallotannin - treated group was significantly lower than that in KA - treated group ( P < 0. 05 ). The expression of PAR was increased in gallotannin - treated group compared with KA - treated group( P < 0. 05 ). AIF in mitochondrial fraction increased and its accumulation in nucleus fraction decreased in gallotannin - treated group compared with KA - treated group ( P <0. 05 ). In addition, gallotannin significantly decreased the protein expression of IL - 1β and TNF - a, which were obviously increased in hippocampus after seizures ( P <0. 05 ). CONCLUSION: PARG inhibition by gallotannin has the neuroprotective effect on the damage of hippocampal neurons induced by seizures. In addition, gallotannin suppresses the translocation of AIF from mitochondria to nucleus and the expression of IL - 1 β and TNF - a in rat hippocampus after seizures.关键词
癫痫/聚腺苷二磷酸核糖糖苷水解酶/单宁酸/海马Key words
Epilepsy/ Poly( adenosine diphosphate ribose ) glycohydrolase/ Gallotannin/ Hippocampus分类
医药卫生引用本文复制引用
王胜军,赵秀鹤,刘学伍,张同霞,陈学兰,迟兆富..聚腺苷二磷酸核糖糖苷水解酶调节癫痫大鼠海马组织凋亡诱导因子及炎症因子的研究[J].中国病理生理杂志,2012,28(2):258-262,5.基金项目
国家自然科学基金资助项目 (No.81000557 （）
No.30876838) （）
