中国病理生理杂志2012，Vol.28Issue(2)：281-286,6.DOI:10.3969/j.issn.1000-4718.2012.02.017
不同缺氧状态对人肝癌HepG2细胞侵袭和转移能力的影响
Effects of hypoxia in different degrees on in vitro invasion and migration of human hepatocarcinoma HepG2 cells
摘要
Abstract
AIM: To study the effects and the underlying mechanisms of hypoxia on adhesion, invasion and migration of HepG2 human liver cancer cells cultured in vitro. METHODS: HepG2 cells were cultured under different concentrations of oxygen for 24 h. MTT assay was used to measure the cell adhesion rate. The Transwell chambers were used to assess in vitro invasion and migration. The expression of CD44v6, matrix metalloproteinase 2( MMP - 2 ), MMP - 9, hypoxia - inducible factor - 1 a( HIF - 1 a) and vascular endothelial growth factor( VEGF ) was detected by the methods of immunocytochemistry, gelatin zymography and RT - PCR. RESULTS: Hypoxia, especially under 3% oxygen, significantly enhanced the adhesion rates of HepG2 cells, and increased the number of the cells migrated through Matrigel - coated chambers ( P <0. 05 ). HepG2 cells treated with 3% and 5% oxygen increased the expression of CD44v6, MMP - 9 , MMP -2, HIF - 1 a and VEGF. CONCLUSION: Pretreatment with moderate hypoxia enhances in vitro adhesion, invasion and migration of HepG2 cells. Multiple molecules including CD44v6, MMP - 2, MMP - 9, HIF - 1 a and VEGF may be involved in the processes.关键词
肝肿瘤/HepG2细胞/缺氧/黏附/肿瘤侵袭Key words
Liver neoplasms/ HepG2 cells/ Hypoxia/ Adhesion/ Neoplasm invasiveness分类
医药卫生引用本文复制引用
余桂芳,严跃红,王瑞鑫,曾文铤,朱科伦..不同缺氧状态对人肝癌HepG2细胞侵袭和转移能力的影响[J].中国病理生理杂志,2012,28(2):281-286,6.基金项目
广东省科技计划项目(No.2010B060900092) （No.2010B060900092）
广州市卫生局中医药科研项目资助(No.2010A34) （No.2010A34）
