中国病理生理杂志2012，Vol.28Issue(2)：298-301,4.DOI:10.3969/j.issn.1000-4718.2012.02.020
黄芪多糖活化AMPK减轻游离脂肪酸对C2C12成肌细胞的细胞毒性
Astragalus polysaccharide attenuates free fatty acid-induced toxicity by activating AMPK in C2C12 myoblasts
摘要
Abstract
AIM: To examine the effects of Astragalus polysaccharide ( APS ) on the toxicity of free fatty acids ( FFAs ) in C2C12 myoblasts. METHODS: C2C12 cells were randomly divided into 5 groups: control group, APS group, 5 - aminoimidazole - 4 - carboxamide - 1 - (3 - D - ribofuranoside ( AICAR )group, FFAs group and FFAs + APS group. MTT assay was used to observe the viability of C2C12 cells. C2C12 cells pretreated with FFAs at concentration of 0. 25 mmol/L for 24 h were exposed to APS at dose of 200 mg/L for 24 h. The expression of total AMP - activated protein kinase ( AMPK ), phosphorylated AMPK ( p - AMPK ) and phosphorylated acetyl - CoA carboxylase ( p - ACC ) was examined by Western blotting. The content of AMP and ATP was determined by HPLC. The structural changes of the mitochondria were examined by transmission electron microscopy. RESULTS: The results of MTT assay indicated that APS improved the viability of C2C12 cells pretreated with FFAs. In FFAs + APS group, the ratio of AMP/ATP was increased after treatment with APS. No difference of total AMPK expression in C2C12 cells between APS group and FFAs group was observed. However, the expression of p - AMPK and p - ACC increased in APS group as compared with FFAs group. The results of transmission electron microscopy indicated that APS improved the vacuolar degeneration of mitochondria resulted from treatment with FFAs in C2C12 cells. CONCLUSION: In C2C12 cells, APS attenuates FFA - induced lipotoxity via a mitochondria - and AMPK - dependent mechanism.关键词
黄芪多糖/游离脂肪酸/AMP激活蛋白激酶/乙酰辅酶A羧化酶/线粒体Key words
Astragalus polysaccharide/ Free fatty acid/ AMP-activated protein kinase/ Acetyl-CoA carboxylase/ Mitochondria分类
医药卫生引用本文复制引用
宋杰,李静,胡阳黔,刘坚,欧阳静萍..黄芪多糖活化AMPK减轻游离脂肪酸对C2C12成肌细胞的细胞毒性[J].中国病理生理杂志,2012,28(2):298-301,4.基金项目
国家自然科学基金资助项目(No.30370673 （）
No.30771023) （）
