中国病理生理杂志2012，Vol.28Issue(2)：314-319,6.DOI:10.3969/j.issn.1000-4718.2012.02.023
IgA肾病组蛋白H3K4三甲基化和DNA甲基化基因修饰的相关性
Correlation of gene modification between histone H3 lysine 4 trimethylation and DNA methylation in IgA nephropathy patients
摘要
Abstract
ADM: To investigate the correlation of gene modification between histone H3 lysine 4 ( H3K4 ) tri-methylation and DNA methylation in IgA nephropathy patients. METHODS: H3K4 trimethylation variations were analyzed in peripheral blood mononuclear cells ( PBMCs ) from 40 IgAN patients and 40 healthy controls by the method of chromatin immunoprecipitation linked to microarrays ( ChIP - chip ). ChIP real - time PCR was used to validate the microarray results. Quantitative real - time PCR ( qRT - PCR ) was employed to analyze mRNA expression. DNA methylation in 4 selected positive genes was analyzed by the method of methylated DNA immunoprecipitation - quantitative PCR ( MeDIP - qPCR ). RESULTS: IgAN patients displayed higher H3K4 trimethylation level and lower DNA methylation level than those of the healthy controls. There were significant differences in DNA methylation and H3K4 trimethylation of 4 selected genes between IgAN patients and the healthy controls ( P <0. 05 ). CONCLUSION: Our studies indicate that significant alterations of H3K4 trimethylation and DNA methylation exist in IgAN patients. There is a correlation of gene modification between DNA mathylation and histone H3 lysine 4 trimethylation in IgAN patients.关键词
IgA肾病/组蛋白H3赖氨酸4/三甲基化/DNA甲基化Key words
IgA nephropathies/ Histone H3 lysine 4/ Trimethylation/ DNA methylation分类
医药卫生引用本文复制引用
齐素文,涂植光,戴勇,睢维国..IgA肾病组蛋白H3K4三甲基化和DNA甲基化基因修饰的相关性[J].中国病理生理杂志,2012,28(2):314-319,6.基金项目
广西壮族自治区自然科学基金资助项目(No.2010GXNSFA013273) （No.2010GXNSFA013273）
