中国病理生理杂志2012，Vol.28Issue(2)：320-325,6.DOI:10.3969/j.issn.1000-4718.2012.02.024
神经生长因子通过TrkANGFR信号途径促进肝细胞增殖
Nerve growth factor promotes proliferation of hepatocytes in vitro via TrkANGFR signal pathway
摘要
Abstract
AIM: To observe the expression and distribution of nerve growth factor ( NGF ), its high - affinity tyrosine kinase A receptor ( TrkANGFR ) and low - affinity p75 neurotrophin receptor ( p75NTR ) in hepatocytes( L02 cells ), and to investigate the effects of exogenous recombinant human NGF - (3 on L02 cells. METHODS: L02 cell line was used in the experiment. The expression and intracellular distribution of NGF, TrkA and p75 were detected by the methods of immunocytochemistry and fluorescent quantitative PCR. The proliferation of L02 cells after exposed to exogenous NGF - (3, anti - NGF, anti - TrkANGFR and anti - p75NTR was detected by XTT { 2,3 - bis( 2 - methoxy - 4 - nitro - 5 - sulfophe-nyl ) - 5 - [ ( phenylamino ) carbonyl ] - 2H - tetrazolium hydroxide } assay. The cell apoptosis and the change of cell cycle of LO2 cells after exposed to exogenous NGF - (3 were determined by flow cytometry with Annexin V - FITC and PI staining. RESULTS: The expression of NGF was mainly localized in the cytoplasm and nuclei of L02 cells. The expression of TrkANGFR was highly and the expression of p75NTR was weakly detected in the cell membrane and cytoplasm of LO2 cells. Exogenous NGF - (3 promoted the expression of NGF and TrkANGFR in LO2 cells. Low dose of exogenous NGF - β ( 12. 5 - 200 μg/L ) promoted L02 cell proliferation and inhibited the cell apoptosis by affecting the cell cycle in S - phase. High dose of NGF - (3 ( > 400 μg/L ) did not promote L02 cell proliferation. Specific neutralizing antibodies of NGF and TrkA decreased the NGF - (3 - induced proliferation of L02 cells. However, blocking the binding of NGF to p75 by anti -p75NTR did not affect the NGF - (3 - induced proliferation of L02 cells. CONCLUSION: L02 cells express NGF, TrkANGFR and p75NTR. Exogenous recombinant human NGF - (3 at optimal dose promotes L02 cell proliferation in a dose -dependent manner possibly via NGF/TrkANGFR signal pathway.关键词
神经生长因子/L02细胞/细胞增殖/细胞凋亡Key words
Nerve growth factor/ L02 cells/ Cell proliferation/ Apoptosis分类
医药卫生引用本文复制引用
李俊峰,舒建昌,陈莲香,朱海燕,吕霞..神经生长因子通过TrkANGFR信号途径促进肝细胞增殖[J].中国病理生理杂志,2012,28(2):320-325,6.基金项目
广东省自然科学基金资助项目(No.9151051501000083) （No.9151051501000083）
