中国病理生理杂志2012，Vol.28Issue(2)：375-379,5.DOI:10.3969/j.issn.1000-4718.2012.02.035
同步监测蟾蜍心脏机械和电活动简便而有效的方法
Simple and effective method for simultaneously detecting the cardiac contractility and electrocardiogram in toads
摘要
Abstract
AIM: To investigate a simple, effective and economic method suitable for simultaneously monitoring the contractility and electrocardiogram ( ECG ) of the in situ spontaneously beating toad hearts. METHODS: The per-fusate was circulated by cannulating in the postcaval vein for inflow and the left branch of the arterial trunk for outflow to detect the cardiac output. Meanwhile, the force transducer was attached on the apex of the heart, and the standard limb lead II was applied to monitor the force and ECG with the BL -420S data acquisition and analysis system. Isoproterenol, a β - adrenoceptor agonist, and its antagonist propranolol, were perfused to confirm the feasibility of the method. RESULTS: When perfused with Ringer's solution, the cardiac output and force were readily detected, and the PR interval, QT interval, and width and amplitude of QRS waves were clear, which was suitable for analyzing the relationship between them. Compared to pretreatment, perfusion of isoproterenol at concentrations of 10-5 ~ 10-4 g/L significantly increased the cardiac output and force, shortened PR interval, and prolonged QT interval ( P < 0. 05, P < 0. 01 ). Propranolol antagonized the positive inotropic effect of isoproterenol. CONCLUSION: This method is suitable for simultaneously detecting the cardiac contractility and ECG in toads.关键词
心脏灌流/心排血量/心电图/蟾蜍Key words
Heart perfusion/ Cardiac output/ Electrocardiogram/ Toads分类
医药卫生引用本文复制引用
李秀国,张民锋,朴日龙,李迎军..同步监测蟾蜍心脏机械和电活动简便而有效的方法[J].中国病理生理杂志,2012,28(2):375-379,5.基金项目
国家教育部留学回国项目(No.教外司留609号) （No.教外司留609号）
