中国病理生理杂志2012，Vol.28Issue(3)：409-414,6.DOI:10.3969/j.issn.1000-4718.2012.03.005
BCL-6在经典型霍奇金淋巴瘤细胞株L428和过表达CD99的L428中的表达差异及其意义
Differential expression of BCL-6 in cell lines of classical Hodgkin's lymphoma L428 and L428
摘要
Abstract
AIM:To invesligale ihe expression of BCL -6 in classical Hodgkin's lymphoma (cHL) cell lines, L428 ( negalive expression of CD99) and L428 - CD99+ ( overexpression of CD99) . METHODS:The expression of CD99 and BCL - 6 al prolein and mRNA levels was delecled in ihe cell lines of L428 and L428 - CD99+ by ihe melhods of immu-nocylochemisLry, confocal immunofluorescence, real - Lime fluorescence quanlilalive PCR and Weslern bloLLing. MTT assay was used Lo delermine ihe difference of proliferation belween ihe cell lines of L428 and L428 - CD99+ . ApopLosis was analyzed by flow cylomelry in ihe cell lines. RESULTS: The resulls of immunocylochemislry and confocal immunofluorescence showed lhal ihe expression of CD99 and BCL -6 was negalive in L428 cell line, bul posilive in L428 - CD99+ cell line. The CD99 was localized in ihe membrane while BCL - 6 expression was in ihe nucleus. The resulls of real - lime fluorescence quanlilalive PCR showed lhal ihe mRNA expression levels of BCL - 6 and CD99 in L428 - CD99+ cells were significantly higher lhan ihose in L428 cells ( P < 0. 01). MTT assay showed lhal ihe proliferation capacity of L428 cells was higher lhan lhal of L428 - CD99+ cells (P < 0. 01) . More apoplolic cells in L428 - CD99+ cell line lhan lhal in L428 cell line ( P < 0. 01) were found. CONCLUSION: The increased expression of CD99 and BCL - 6 may lead lo lower proliferation capacity and higher apoplolic rale of cHL, which are expecled lo be helpful in prevenlion and Irealmenl of cHL.关键词
L428细胞株/CD99/BCL-6Key words
L428 cell line/ CD99/ BCL - 6分类
医药卫生引用本文复制引用
李锋,黄作平,黄学平,王志强,钟琳,周新华,吴自勍,朱梅刚,赵彤..BCL-6在经典型霍奇金淋巴瘤细胞株L428和过表达CD99的L428中的表达差异及其意义[J].中国病理生理杂志,2012,28(3):409-414,6.基金项目
国家自然科学基金资助项目(No.30770908 （）
No.81071941) （）
