中国病理生理杂志2012，Vol.28Issue(3)：453-458,6.DOI:10.3969/j.issn.1000-4718.2012.03.012
心肌再灌注对抑郁大鼠心肌细胞凋亡以及bcl-2、bax和caspase-3表达的影响
Effect of myocardial reperfusion on cardiocyte apoptosis and expression of bcl-2, bax and caspase-3 in rats with depression
摘要
Abstract
AIM:To explore ihe effecl of ischemia/reperfusion (I/R) on cardiac myocyle apoplosis and ihe expression of bcl -2, bax and caspase - 3 in ihe rals wilh depression. METHODS; Thirty - Lwo Wislar rals were randomly divided inlo 4 groups,including group A; sham operation group; group B; ihe rals wilh depression undergoing sham opera-lion; group C; ihe rals wilh myocardial I/R operalion; group D; ihe rals wilh depression undergoing myocardial I/R opera-lion. The ral model of depression was produced by chronic mild unpredictable slress and separalion. The behaviors of ihe rals were delecled by open field lesl and fluid consumption lesl. The myocardial I/R model was made by ligaling ihe lefl an-lerior descending branch of coronary artery in ihe rals. The apoplolic cardiomyocyles were delecled by in siLu TdT - media-led dUTP nick end labeling (TUNEL) melhod, and ihe expression of bcl -2, bax and caspase - 3 was delemined by ihe melhods of immunohislochemislry and reverse Iranscriplion polymerase chain reaction ( RT - PCR) . RESULTS; Compared wilh group A and group B, ihe numbers of apoplolic cardiomyocyles in group C and group D were significantly increased (P < 0. 01) , and ihe expression of bcl - 2, bax and caspase - 3 in group C and group D was also significantly increased ( P < 0. 01). No significant difference between group A and B was observed. Compared wilh group C, the number of apoplolic cardiomyocyles in group D was significantly increased (P < 0. 05). The gene expression of bcl -2 in group D was decreased significantly ( P < 0. 05 ) , while the gene expression of bax and caspase - 3 in group D was significantly increased ( P < 0. 05 ) . CONCLUSION; Myocardial reperfusion increases apoptosis in ischemic cardiomyocyles in the rals with depression. The mechanisms may be associated with up - regulaling the gene expression of bax and caspase - 3 while down - regulaling bcl - 2 expression.关键词
心肌再灌注/抑郁/大鼠/细胞凋亡/基因表达Key words
Myocardial reperfusion/ Depression/ Rats/ Apoptosis/ Gene expression分类
医药卫生引用本文复制引用
刘淑珍,尤鑫,熊小栓,刘兴德..心肌再灌注对抑郁大鼠心肌细胞凋亡以及bcl-2、bax和caspase-3表达的影响[J].中国病理生理杂志,2012,28(3):453-458,6.基金项目
贵州省优秀科技教育人才省长资金项目(2005)306号 （2005）
