中国病理生理杂志2012，Vol.28Issue(3)：478-482,5.DOI:10.3969/j.issn.1000-4718.2012.03.016
脂氧素A4抑制内毒素诱导的人支气管上皮细胞环氧合酶2及前列腺素E2的表达
doi:10.3969/j.issn.1000-4718.2012.03.016Lipoxin A4 reduces lipopolysaccharide-induced expression of cyclooxygenase 2 and prostaglandin E2 in human bronchial epithelial cells
摘要
Abstract
AIM:To explore ihe effecls of lipoxin A4 on ihe expression of cyclooxygenase 2 ( COX - 2) in human bronchial epilhelial cells (HBECs) . METHODS; HBECs were incubaled wilh various concenlralions (0. 1, 1 and 10 mg/L) of lipopolysaccharide( LPS) for 9 h, or 1 mg/L LPS for different Lime (3 h, 6 h and 9 h) . The levels of COX - 2 mRNA in HBECs and proslaglandin E2 ( PGE2 ) in ihe cullure supernalanl were measured. In addition, lhe HBECs were exposed lo lipoxin A4 al concentration of 0, 100 and 400μmol/L after stimulated with LPS at concentration of 1 mg/L for 9 h, and the supernatant of the culture cells was collected for determining the content of PGE2 by ELISA. The cells were also harvested, and the mRNA and protein levels of COX -2 were analyzed by RT - PCR and Western blotting, respectively. RESULTS: LPS increased the mRNA expression of COX - 2 and production of PGE2 in a dose and time dependent manners in HBECs. Induction of COX - 2 mRNA and protein by LPS were inhibited by lipoxin A4 in a dose - dependent manner. Lipoxin A4 also significantly decreased LPS - induced production of PGE2. CONCLUSION: Lipoxin A4 down - regulates LPS - induced expression of COX - 2 and consequently inhibits the production of PGE2 in HBECs.关键词
脂氧素A4/人支气管上皮细胞/环氧合酶2/脂多糖类Key words
Lipoxin A4/ Human bronchial epithelial cells/ Cyclooxygenase 2/ Lipopolysaccharides分类
医药卫生引用本文复制引用
朱天琦,郑声星,金胜威..脂氧素A4抑制内毒素诱导的人支气管上皮细胞环氧合酶2及前列腺素E2的表达[J].中国病理生理杂志,2012,28(3):478-482,5.基金项目
国家自然科学基金资助资助项目(No.81070061) （No.81070061）
浙江省自然科学基金项目(No.Y2100885) （No.Y2100885）
浙江省卫生高层次创新人才培养项目 （）
