中国病理生理杂志2012，Vol.28Issue(3)：483-487,5.DOI:10.3969/j.issn.1000-4718.2012.03.017
类风湿关节炎患者外周血单个核细胞Sonic Hedgehog信号通路表达的初步研究
Preliminary study of Sonic Hedgehog signaling pathway in rheumatoid arthritis
摘要
Abstract
AIM: To invesligale lhe expression of Sonic Hedgehog (Shh) signaling palhway - associaled fac-Lors in peripheral blood mononuclear cells (PBMCs) and synovial lissues of rheumaloid arlhrilis (RA). METHODS" The mRNA expression levels of Shh, Plchl and Glil in PBMCs of 35 RA palienls, and 35 age - and sex - malched heallhy con-Lrols were analyzed by real - Lime PCR. The expression of Shh, Plchl and Glil in synovial Lissues was delecled by immuno-hislochemisly assay in 10 RA palienls and 5 palienls wilh Iraumalic or meniscal injury (no arlhrilis) as conlrol group. All palienls accorded wilh lhe American College of Rheumatology ( ACR) 1987 revised classification criteria for determining RA, and lhe score of DAS28 was ≥3. 2. RESULTS: The results of real - lime PCR showed thai the expression of Shh and Glil mRNA in RA palienls was higher lhan thai in the controls (Shh and Glil in RA were 1.36 ±1.48 and 1. 15 ±0.68, while Shh and Glil in conlrol group were 0. 47 ± 0. 25 and 0. 49 ± 0. 05 , respectively) . The mRNA expression of Plchl be-tween lhe 2 groups had no significant difference. Similarly, lhe results of immunohislochemislry assay showed lhat lhe positive slaining rates of Shh and Glil in RA group were higher lhan those in conlrol group. However, no difference of Plchl posilive slaining rale between lhe 2 groups was observed ( P > 0. 05 ) . CONCLUSION: The positive expression of Shh and Glil indicates the aclivation of Shh signaling palhway in the RA palienls.关键词
关节炎/类风湿/Sonic Hedgehog通路/单核细胞/滑膜Key words
Arlhrilis, rheumaloid/ Sonic Hedgehog pathway/ Monocytes/ Synovial membrane分类
医药卫生引用本文复制引用
王明霞,黄建林,朱尚玲,彭蔚湘,谢宝钊,林灼锋,古洁若..类风湿关节炎患者外周血单个核细胞Sonic Hedgehog信号通路表达的初步研究[J].中国病理生理杂志,2012,28(3):483-487,5.基金项目
国家自然科学基金资助项目(No.81072480) （No.81072480）
广东省自然科学基金资助项目(No.10151008901000210) （No.10151008901000210）
