中国草食动物2011，Vol.31Issue(6)：5-8,4.
山羊Ets-1基因cDNA的克隆与序列分析
Cloning and Sequence Analysis ofEts-1 Gene in Goats
摘要
Abstract
According to the highly conservative region of Ets-l gene sequences from bovine (Bos taunts),human (Homo sapiens) and mouse (Mus musculus) in GenBank.the specific primers were designed to clone the cDNA region of Ets-l gene in mammary gland of Xinong Saanen dairy goats using RT-PCR and RACE (rapid amplification cDNA ends) methods,the sequence analyses of nucleotide sequence,and the deduced amino acid sequence of Ets-l gene were conducted. The results showed that the full length of Ets-l gene was 2 263 bp(GenBank accession No.HQ589338), containing 331 bp of 5'UTR,606 bp of 3"UTR,and 1 326 bp of coding sequences (CDS),which encoded a protein of 441 amino acid residues. The nucleotide sequence alignment indicated that the similarities of coding region of goat Ets-l were 98 %,94 %,92 % and 90 %;the similarities of 5 'UTR were 98 %, 85 %, 82 % and 71 %; the similarities of 31JTR were 96 %, 83 %, 81 % and 77 %, compared to those of bovine,porcine,human and rat,respectively. The AA sequence alignment indicated that the protein sequence of goat Ets-l shared high similarities (more than 95 % )with bovine,porcine,human and rat .respectively. The protein molecular weight was 50 340.8 D and the isoelectric point was 5.08. The protein of Ets-l had a helix-turn-helix structure. No transmembrane structure was speculated and no signal peptide was found in the entire sequence. Thus, it can be concluded that the full length cDNA of goat Ets-l gene has been successively cloned.关键词
山羊/Ets-1/基因克隆Key words
goat/ Ets-l/ gene cloning分类
农业科技引用本文复制引用
李君,罗军,许会芬,胡仕良..山羊Ets-1基因cDNA的克隆与序列分析[J].中国草食动物,2011,31(6):5-8,4.基金项目
转基因生物新品种培育重大专项(2009ZX08009-162B) （2009ZX08009-162B）
公益性行业(农业)科研专项经费项目(201103038) （农业）
