中国当代儿科杂志2011，Vol.13Issue(12)：989-994,6.
低剂量毒死蜱暴露对新生大鼠黑质多巴胺能神经元发育及行为的影响
Effects of low-dose chlorpyrifos exposure on dopaminergic neurons in the midbrain substantia nigra and neural behavioral development in neonatal rats
摘要
Abstract
Objective To explore the effects of low-dose chlorpyrifos ( CPF) exposure on dopaminergic ( DA) neurons in the midbrain substantia nigra and neural behavioral development in neonatal rats. Methods Postnatal 11 day old Sprague-Dawley rats were randomly assigned into CPF, menstruum dimethysulibxide ( DMSO) and normal saline ( NS) groups. The rats in the CPF group were injected with low-dose CPF (5 mg/kg·d) on postnatal days 11-14. The two control groups were injected with DMSO or NS respectively. The rats were sacrificed on postnatal days 15, 20, 30, and 60. Body weight gain, outward appearance of brain tissue, the coefficient of brain and the water content of brain tissue were measured. Tyrosine hydroxylase ( TH) expression in DA neurons in the midbrain substantial nigra was examined by immunohistochemical straining. Immune electron microscopy was used to examine the subcellular structure of DA neurons. Open field test, grip strength test, slope test and Morris water maze test were used to examine the neurobehavioral changes. Results The outward appearance of brain tissue was normal in the three groups. There were no significant differences in the absolute value of body weight gain, the coefficient of brain and the water content of brain tissue among the three groups. CPF exposure decreased the level of TH immunoreactivity ( P <0. 05 ) in the substantia nigra of CPF group since postnatal day 30 compared with the DMSO and NS groups. The subcellular structures of some DA neurons in the CPF group were impaired. Decreased motor activity and learning and memory impairments were observed in the CPF group compared with those in the DMSO and NS groups (P < 0. 05) since postnatal day 30. Conclusions CPF exposure during the neonatal period can cause long-term motor activity and learning and memory impairments in accompany with DA neurons damage in the midbrain substantia nigra.关键词
毒死蜱/多巴胺能神经元/神经行为/大鼠Key words
Chlorpyrifos/ Dopaminergic neuron/ Neural behavior/ Kats分类
医药卫生引用本文复制引用
张洁,赵玲玲,胡治平,周军,邓礼,谷芬,戴红梅,黄敏..低剂量毒死蜱暴露对新生大鼠黑质多巴胺能神经元发育及行为的影响[J].中国当代儿科杂志,2011,13(12):989-994,6.基金项目
湖南省自然科学基金(0CJJ5043) （0CJJ5043）
湖南省社会发展基金(02ssy3078) （02ssy3078）
长沙市科技局基金(K1003053-31). （K1003053-31）
