中国动物检疫2011，Vol.28Issue(9)：31-34,4.
西尼罗河病毒病竞争ELISA检测方法的初步建立及应用
Development and Application of a Competitive Enzyme-Linked lmmtmosorbent Assay for the Detection of Antibody against West Nile Virus
常娓娓 1王淑娟 2吴晓东 1刘华雷 1赵永刚 1王志亮1
作者信息
- 1. 中国动物卫生与流行病学中心国家外来动物疫病诊断中心,山东青岛266032
- 2. 扬州大学兽医学院,江苏扬州225009
- 折叠
摘要
Abstract
A cELISA for detection of WNV antibody was established using purified WNV E protein domain IH as antigen coating the ELISA plate and monoclonal antibody 8F4A4 specific to WNV as competitive detection antibody.The results showed that the antigen coat concentration was 530 ng/mL, the best dilution of McAb 8F4A4 was 1：8000 and the best serum dilution was I：10.A total of 56 clinically confirmed negative control sera were tested by the cELISA.Through analysis, it was concluded that the cut-off value was 30%.A set of 220 clinical serum samples were tested by the cELISA and all were negative.Contrast to the commercial kits, the method could differentiate the JEV and WNV antibody, providing an efficient antibody detection method for the West Nile virus disease epidemiology investigation in our country.关键词
西尼罗河病毒/囊膜蛋白/单克隆抗体/竞争ELISAKey words
West Nile virus （WNV）/Envelope protein/Monlclonal antibody/cELISA分类
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常娓娓,王淑娟,吴晓东,刘华雷,赵永刚,王志亮..西尼罗河病毒病竞争ELISA检测方法的初步建立及应用[J].中国动物检疫,2011,28(9):31-34,4.
