中国动物检疫2011，Vol.28Issue(11)：39-43,5.
多重PCR方法检测锦鲤疱疹病毒基因
A Multiplex PCR Assay for Simultaneous Detection of Koi Herpesvirus Gene
摘要
Abstract
A multiplex polymerase chain reaction （multi-PCR） was developed for simultaneously detection of 3 KHV viral DNA segments to improve the diagnosis of KHV infection.3 sets of primers were designed targeting specific sequences of KHVSphI（AY568590）,KHV5/9（AF411803）and KHV TK gene（AJ535112）,which were frequently used in PCR assay for detecting KHV viral DNA used in the assay and each of them could amplify and result in 290 bp,484 bp and 409 bp fragment with viral nucleic acids by PCR products with different size,the size of KHVSphI,KHV5/9and KHV TK gene.They were highly specific and no specific bands of the same size were amplified from SVCV rival cDNA.The sensitivity of the multiplex PCR was 10fg for KHVSphI,100 fg for KHV5/9and KHV TK gene.In the field application,the results were consistent with those results of the single PCR detection,indicating that this multi-PCR method was superior in terms of sensivity,specificity,rapidity and simplicity,and potentially a valuabable diagnostic tool for KHV infections.关键词
检测/锦鲤疱疹病毒/多重PCRKey words
Detection/Kio herpesvirus/Multiplex PCR分类
农业科技引用本文复制引用
乌日琴,陈芳,张艺宜,刘芸莉,刘中勇,林志雄..多重PCR方法检测锦鲤疱疹病毒基因[J].中国动物检疫,2011,28(11):39-43,5.基金项目
国家质量监督检验检疫总局科技项目（国检科2007IK014)资助 （）
