中国动物检疫2011，Vol.28Issue(12)：39-42,4.
绵羊细粒棘球蚴全血金标渗滤检测方法的建立
Development of Dot Immunogold Filtration Assay （DIGFA） for Rapid Detection of Sheep Echinococcus granulosus Antidody with Whole Blood Sample
摘要
Abstract
In order to establish a rapid and high-performance immunoassay assay in whole blood of livestock for diagnosis of hydatidosis, the cyst fluid antigen was extracted from fresh sheep Echinococcus cysts and salted-out by ammonium sulphate.The soluble cyst fluid used as antigen was dotted on nitro-cellulose and colliodal gold-donkey anti-goat IgG and colliodal gold-rabbit anti-mice IgG were employed as the probe, and a filtration device with vertical flow was applied to detect the specific IgG antibodies of sheep and mice infected with protoscoleces of Echinococcus granulosus. By using the new immunoassay, it was found that 90.91%-94.4% of whole blood and serum from sheep and 100% of whole blood and serum from mice were positive for hydatidosis, and 5% of health individuals had false positive results.However, partially high percentages of cross reactions could be demonstrated in serum of sheep with Taenia multiceps.The results showed that the dot immune gold filtration assay （DIGFA） could be used for diagnosis and quarantine inspection of livestock hydatidosis.关键词
细粒棘球蚴/囊液抗原/金标免疫渗滤法/免疫诊断/绵羊Key words
Echinococcusgranulosus/cyst fluidantigen/DIGFA/immunodiagnosis/sheep分类
农业科技引用本文复制引用
余华,聂华明,严玉宝,杨光友,陈世界,胡娟,崔鹏博..绵羊细粒棘球蚴全血金标渗滤检测方法的建立[J].中国动物检疫,2011,28(12):39-42,4.基金项目
国家质检总局科技计划项目“动物包虫病检验检疫方法研究与规范”（2009IK019) （）
