中国骨伤2011，Vol.24Issue(12)：1024-1027,4.DOI:10.3969/j.issn.1003-0034.2011.12.014
体外负压培养对骨髓间充质干细胞成骨活性的影响
Effects of negative pressure on osteogenesis in human bone marrow-derived stroma cells cultured in vitro
摘要
Abstract
Objective:To investigate effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells (BMSCs) cultured in vitro. Methods:The third passage cells were divided into negative pressure treatment group and control group. The cells in the treatment group were induced by negative pressure intermittently (pressure: 17 kPa, 30 min per time, and four times of each day). The cells in the control group were cultured in conventional condition. The osteogenesis of BMSCs was examined by phase-contrast microscopy. The alkaline phosphatase (ALP) activities were determined. The expression of collagen type I was detected by immunohistochemistry method. The Mrna expressions of osteoprotegerin (OPG) and osteoprotegerin ligand (OPGL) in BMSCs were analyzed by real-time polymerase chain reaction (PCR). Results: BMSCs showed a typical appearance of osteoblast after 2 weeks of induction by intermittent negative pressure. The activity of ALP increased significantly, and the expression of collagen type I was positive. In the treatment group, the Mrna expression of OPG increased significantly (P<0.05) and the Mrna expression of OPGL decreased significantly (P<0.05) after 2 weeks, compared with the control. However, 3 days after the exposure to 2-week negative pressure, these were no significantly different from that of the control group(P>0.05). Conclusion: Intermittent negative pressure could promote osteogenesis in BMSCs in vitro.关键词
间充质干细胞/骨髓/成骨细胞/细胞培养技术Key words
Mesenchymal stem cells/ Bone marrow/ Osteoblasts/ Cell culture techniques引用本文复制引用
杨治,朱养均,程延,尚保生,常瑞,何鹏,张银刚..体外负压培养对骨髓间充质干细胞成骨活性的影响[J].中国骨伤,2011,24(12):1024-1027,4.基金项目
国家自然基金资助项目(编号:30973063) （编号:30973063）
陕西省卫生厅科研基金(编号:2010D23) （编号:2010D23）
西安市卫生局科技立项(编号:J2010B31) （编号:J2010B31）
