中国康复理论与实践2012，Vol.18Issue(1)：33-36,4.DOI:10.3969/j.issn.1006-9771.2012.01.011
电刺激对大鼠脊髓损伤后神经生长因子表达的影响
Effects of Electrical Stimulation on Expression of Nerve Growth Factor in Adult Rats with Spinal Cord Injury
摘要
Abstract
Objective To investigate the effects of electrical stimulation on the expression of nerve growth factor (NGF) in adult rats with spinal cord injury. Methods 72 adult SD rats were randomly divided into 3 groups: damage control group, electrical stimulation group and normal group. The spinal cord incomplete injury model on T9 was made with Allen's method. The electrical stimulation group received electrical stimulation for 7 d. They were assessed with the Basso, Beattie & Bresnahan locomotor rating scale (BBB scale), and the expression of NGF were tested with immunohistochemistry and Western blot 1 d, 3 d, 5 d, 7 d after injury. Results The BBB score improved after spinal cord injury, and electrical stimulation group improved more than control group since the 5th day after injury (P<0.05). The expression of NGF increased in electrical stimulation and injury control group after injury (P<0.05). Conclusion Electrical stimulation after spinal cord injury induces expression of NGF that creates a favorable microenvironment for nerve regeneration.关键词
脊髓损伤/神经生长因子/电刺激Key words
spinal cord injury/ nerve growth factor/ electrical stimulation分类
医药卫生引用本文复制引用
陈虹,李俊岑,党彦丽,刘姿辰,杨拯,张晓..电刺激对大鼠脊髓损伤后神经生长因子表达的影响[J].中国康复理论与实践,2012,18(1):33-36,4.基金项目
四川省科技厅课题(2008SZ0053) （2008SZ0053）
成都医学院创新实验项目(CX2010035). （CX2010035）
