中国临床药理学与治疗学2011，Vol.16Issue(12)：1340-1346,7.
脑复合剂对创伤性脑损伤模型大鼠脑内NO、nNOS活性及表达的影响
Effects of compound decotion on activity and expression of nitric oxide and neuronal nitric oxide synthase in the brain of traumatic brain injury rat model
摘要
Abstract
AIM:To explore the possible mechanism of the neuroprotective effect of compound decotion on traumatic brain injury model. METHODS: The traumatic brain injury rat model was induced by improved Feeneys fall weight method and divided into sham operation group, TBI model group and compound decotion treatment group, compound decotion treatment group rats were intragastrically administered with compound decotion at 10 g . Kg-1 . D-1. Sham operation group and TBI model group were treated with same dose of normal saline, twice per day for seven days. The brains were taken out at 24, 72 h and one week after injury, respectively. The morphological changes were observed by HE staining in the cerebral cortex. The NO and NOS concentration and neuronal nitric oxide synthase (nNOS) activity were detected by neurochemistry stain method. nNOS expression were detected by immunohistochemical method. nNOS-mRNA expression were detected by in situ hybridization. RESULTS:In TBI mod-el group, the concentration of NO and NOS and the activity and expression of nNOS and the expression of nNOS- mRNA were markedly elevated at different degrees at different time point and especially 24 hours after injury and still higher than those of sham operation group one week after injury. However, compared with the TBI model group, the concentration of NO and NOS and the activity and expression of nNOS and the expression of nNOS-mRNA in compound decotion treatment group were obviously decreased and reach normal level one week after injury (P <0. 05). CONCLUSION: NO and nNOS play an important role in the pathophysiological process of TBI. Inhibition of activated NO and nNOS and mitigate secondary brain injury may represent a potential neuroprotective mechanism for the treatment of TBI with compound decotion.关键词
创伤性脑损伤/一氧化氮/神经元型一氧化氮合酶Key words
Traumatic brain injury/Nitric oxide/Neuronal nitric oxide synthase分类
医药卫生引用本文复制引用
苗静琨,陈启雄,吴小玫,刘振球,张晓萍..脑复合剂对创伤性脑损伤模型大鼠脑内NO、nNOS活性及表达的影响[J].中国临床药理学与治疗学,2011,16(12):1340-1346,7.基金项目
重庆市卫生局中医药科研资助项目(渝中医20024270) （渝中医20024270）
