中国临床药理学与治疗学2011，Vol.16Issue(12)：1367-1373,7.
冠欣舒胶囊对大鼠心肌细胞缺氧/复氧损伤保护作用的拆方实验研究
Experiment study of Guanxinshu capsule attenuates anoxia/reoxygenation injury in neonatal rat cardiomyocytes by partial formulation analysis
摘要
Abstract
AIM: To investigate the protective effect of guanxinshu capsule on cardiomyocytes after anoxia-reoxygenation(A/R) injury, partial formulation experiment was executed by orthogonal design in order to obtain the best ratio of Guanxinshu. METHODS: Based on the effective site and the basic ratio isolated from original chemical formulation of salvia miltiorrhiza, pa-nax pseudo-ginseng, lignum acronylchiae, orthogonal table was designed. The primarily neonatal rat ventricular cardiomyocytes were randomly divided into 18 groups- (l)control group; (2)A/R group; (3) - (18) experimental groups treated with different ratio; Cell viability, SOD, Catalase, GPx activities, MDA contents and LDH activitied were detected. Best ratio of program was obtained by analysis of integrated e-quilibrium and then verificated. RESULTS: These groups treated with different ratio of Guanxinshu elevated cell viability and SOD, Catalase, GPx activity, whereas LDH activitiesand MDA contents reduced ( P < 0. 01 or P < 0. 05 ) ; Panax pseudo-ginseng saponins and salvia miltiorrhiza phenolic acid have played an important role of protection of cardiomyocyte and increased ability of attenuation lipid peroxida-tion; The best ratio of Guanxinshu is: A3B3C2D4. CONCLUSION: The different ratio of Guanxinshu have protected cardiomyocyte a-gainst A/R injury, the mechanism may be relative with attenuation lipid peroxidation. The best ratio of Guanxinshu is: salvia miltiorrhiza total phenolic acid 0. 027 mg, Panax pseudo-ginseng total saponins 0. 048 mg, lignum acronylchiae oil 0.002625 mg, lignum acronylchiae total fla-vonoids 0. 01326 mg.关键词
冠欣舒胶囊/心肌细胞/缺氧/复氧损伤/正交设计/拆方实验Key words
Guanxinshu/Cardiomyocyte/Anoxia-reoxygenation(A/R) injury/Orthogonal design/Partial formulation experiment分类
医药卫生引用本文复制引用
何欢,侯莉,黄璜,王金钱,范玫玫,何明..冠欣舒胶囊对大鼠心肌细胞缺氧/复氧损伤保护作用的拆方实验研究[J].中国临床药理学与治疗学,2011,16(12):1367-1373,7.基金项目
科技部国际科技合作项目"中药有效部位复方制剂冠欣舒的研究与开发"(2006DFB33080) （2006DFB33080）
