中国临床药理学与治疗学2012，Vol.17Issue(1)：25-29,5.
CTP-HBcAg18-27-Tapasin融合蛋白胞内转导功能的检测
Transmission functions of fusion protein CTP-HBcAg18-27-Tapasin in the cells
摘要
Abstract
AIM: To observe the function of transduction of fusion protein CTP-HBcAgl8-27-Tapasin in antigen presenting cells. METHODS: Bone marrow derived DCs isolated from BALB/c mice were cultured with recombinant granulocyte-macrophage colony-stimulating factor and recombinant interleutin-4 for 5 days followed by LPS added to induce DCs maturation. Then, different dosages of CTP-HBcAgl8-27-Tapasin and RPMI-1640 were added to cell culture medium. Subsequently, the distribution and localization of intracellular immunofluores-cence were observed by confocal microscopy with fluorescence intensity assaying quantitatively. Futhermore, the transduction efficiency of CTP-HBcAgl8-27-Tapasin was evaluated by detecting the differences of expression of Tapasin using western blot in different group. RESULTS: DCswere cultured and identified successfully; Immu-nofluorescence confirmed the CTP-HBcAgl8-27-Tapasin could penetrate the membrane into the cytoplasm of DCs but not into the nucleus. Fluorescence intensity of 50 μg/L CTP-HBcAgl8-27-Tapasin group were higher than 10 μg/L CTP-HBcAgl8-27-Tapasin group and blank groups. Western blot also confirmed that CTP-HBcAgl8-27-Tapasin can penetrate the cell membrane into the DCs, and the data showed the difference was statistical. CONCLUSION: CTP-HBcAgl8-27-Tapasin has the ability of penetrating and locating in the cytoplasm of DCs.关键词
CTP/HBcAg18-27/Tapasin/转导/树突状细胞Key words
CTP/ HBcAgl8-27/ Tapasin/ Transduction/ Dendritic cells分类
医药卫生引用本文复制引用
刘红红,陈小华,周丽芹,刘雪妮,余永胜,臧国庆,汤正好..CTP-HBcAg18-27-Tapasin融合蛋白胞内转导功能的检测[J].中国临床药理学与治疗学,2012,17(1):25-29,5.基金项目
国家自然基金项目(31000414) （31000414）
