中国临床药理学与治疗学2012，Vol.17Issue(1)：40-46,7.
青蒿琥酯增加小鼠腹腔巨噬细胞内化内毒素、大肠埃希菌的作用及可能机制
Artesunate enhance the ability of peritoneal macrophages endocytosing LPS and Escherichia coli and its mechnisms
摘要
Abstract
AIM: To investigate the effect of artesunate (AS) on peritoneal macrophages en-docytosing lipopolysaccharide/endotoxin (LPS) and phagocytosing Escherichia coli. METHODS: Effects of various concentrations of monodansyl-cadaverine (MDC) and chloroquine(CQ) on the viability of peritoneal macrophages were observed by MTT test. Effects of artesunate, monodansylcadaverine and chloroquine on LPS internalization of peritoneal macrophages were detected by confocal laser scaning microscopy. The effect of artesunate on Escherichia coli internalization of peritoneal macrophages was examined by confocal laser scaning microscopy and bacterial clone forming counting test. The effect of artesunate on class A scavenger receptor (SR-A) mRNA expression was measured using RT-PCR method. RESULTS:25 Mg /mL of MDC and 20 fj.g /mL of chloroquine had no effect on the viability of peritoneal macrophages. Artesunatecould increase LPS internalization of peritoneal macrophages, while MDC and chloroquine could inhibit LPS but artesunate could still increase LPS internalization of macrophages treated with MDC and chloroquine. Artesunate could increase Escherichia coli internalization, and artesunate could up-regulate SR-A mRNA expression of peritoneal macrophages treated with or without LPS. CONCLUSION: Artesunate could enhance the ability of the peritoneal macrophages to internalize LPS and Escherichia coli, which was associated with up-regulation of SR-A mRNA expression of the peritoneal macrophages.关键词
青蒿琥酯/内化/脂多糖/大肠埃希茵/清道夫受体Key words
Artesunate/ Internalization/ Li-popolysaccharide/ Escherichia coli/ Scavenger receptor分类
医药卫生引用本文复制引用
余梦辰,潘夕春,李小丽,李军,肖康康,李斌,周红..青蒿琥酯增加小鼠腹腔巨噬细胞内化内毒素、大肠埃希菌的作用及可能机制[J].中国临床药理学与治疗学,2012,17(1):40-46,7.基金项目
国家自然科学基金青年科学基金项目(81001440) （81001440）
