中国临床药理学杂志2012，Vol.28Issue(1)：40-42,3.
紫杉醇对血管外膜成纤维细胞增殖和迁移的影响
Effects of paclitaxel on migration and proliferation of vascular adventitial fibroblasts
摘要
Abstract
Objective To study on the migration and proliferation of vascular adventitial fibroblasts. Methods Adventitial fibroblasts ( AF) were isolated from thoracic aortas of male Sprague - Dawley rats. The proliferation of adventitial fibroblasts was examined on the proliferating cell nuclear antigen ( PCNA) by immunofluorescence staining. Analysis of the cell cycle is performed by flow cytometry. The ability of cell migration was detected by chamber invasive models. Results Low dose pacli-taxel ( ≤10 nmol · L-1) for 12 hours have no significant decrease in AF survival rate when compared with control group ( P < 0. 05 ). The percentage of G0 - G1 phase cells increased while the G2 - M and S phase decreased after incubation with paclitaxel (9 nmol · L-1) for 24 hours ( P <0. 05). Paclitaxel significantly inhibit the PCNA expression as well as the migration ability in AF(P <0. 01). Conclusion Low dose of paclitaxel evidently inhibit the proliferation and migration of AF, therefore inhibit restenosis after coronary stenting.关键词
血管外膜成纤维细胞/紫杉醇Key words
adventitial fibroblast/ paclitaxel分类
医药卫生引用本文复制引用
沈凯,张怀勤,袁国裕,顾磊科..紫杉醇对血管外膜成纤维细胞增殖和迁移的影响[J].中国临床药理学杂志,2012,28(1):40-42,3.基金项目
浙江省医药卫生科学研究基金资助项目(2007A198) （2007A198）
浙江省中医药科技计划基金资助项目(2007CA129) （2007CA129）
