中国临床药理学杂志2012，Vol.28Issue(1)：49-52,4.
参麦注射液和注射用血塞通对大鼠肝脏及肠道药物代谢酶CYP450的影响
Impact of Shenmai injection and Xuesaitong injection on CYP450s in rat liver and intestine after successive administration
摘要
Abstract
Objective To investigate the influence of Shenmai injection (SM) and Xuesaitong injection (XST) on drag metablic enzyme of cyto-chrome P450(CYP450) system in rat. Methods Microsome of rat liver and intestine was prepared after intravenous injection of SM and XST for 10 days. The content of total CYP450s in rat liver microsome was measured by UV - visible absorption spectrometry. Meanwhile, the protein expression of CYP1A2, CYP2C11, CYP2E1 and CYP3A in the liver micro-some and CYP3A in the small intestine microsome were detected by western blotting analysis. Results Although no significant difference of total P450 content was observed between each treatment group and control group (P > 0.05), but obvious difference was exhibited in western bloting analysis on CYPs. In addition to both SM and XST induced the CYP2E1 in liver microsome significantly, SM inhibited the CYP3A in intestine microsome while XST induced the expression of CYP1A2, CYP2C11 and CYP3A in liver microsome significantly. Conclusion Since the expression of CYPs subtypes in liver and intestine was significantly changed after successive administration, the possible side effect of drug - drug interaction could not be neglected in the period of clinical therapy.关键词
参脉注射液/注射用血塞通/细胞色素P450/蛋白免疫印迹法/药物相互作用Key words
Shenmai injection/ Xuesaitong injection/ CYP450/ Western blot/drug interactions分类
医药卫生引用本文复制引用
卜明华,郑咏秋,张颖,李利群,刘建勋,刘东春..参麦注射液和注射用血塞通对大鼠肝脏及肠道药物代谢酶CYP450的影响[J].中国临床药理学杂志,2012,28(1):49-52,4.基金项目
国家自然科学基金资助项目(30701101) （30701101）
