中国临床药理学杂志2012，Vol.28Issue(1)：56-58,3.
HPLC法测定血浆和尿液中头孢拉宗浓度
Determination concentration of cefbuperazone in human plasma and urine by HPLC
梅和坤 1白楠 1梁蓓蓓 1蔡芸 1王睿1
作者信息
- 1. 解放军总医院临床药理研究室,北京100853
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determining cef-buperazone concentration in human plasma and urine. Methods With levofloxacin as internal standard, plasma samples extracted by perchloric acid. Results Determination of cefbuperazone has good linearity in the 2 - 250 and 5 - 500 μg · mL-1, lower limit of quantification was 2 μg · mL-1and 5 μg · mL-1, extraction recovery is 68.8% to 77.4% and 89. 7% to 102. 2% , accuracy was 95. 4% to 108. 8% and 86. 5% to 108. 7% , and precision of plasma(RSD) ≤3. 9% and precision of urine ≤9. 1%. Conclusion The established HPLC method is specific, sensitive , and simple, fast, accurate and suitable for determination of cefbuperazone in human plasma and urine.关键词
头孢拉宗/高效液相色谱法/血药浓度Key words
cefbuperazone/ HPLC/ plasma concentration分类
医药卫生引用本文复制引用
梅和坤,白楠,梁蓓蓓,蔡芸,王睿..HPLC法测定血浆和尿液中头孢拉宗浓度[J].中国临床药理学杂志,2012,28(1):56-58,3.
