中国临床医学2011，Vol.18Issue(6)：757-760,4.
Stealth核糖核苷酸干扰抑制胰腺癌细胞DNMT1表达的研究
Study of DNMT1 Stealth RNA Interference on the Expression of DNMTI Gene in the Pancreatic Cancer Cell Line
摘要
Abstract
Objective:To investigate the suppression effect of the stealth RNAi targeting DNMT1 gene by transecting the pancreatic cancer cell line. Methods: The DNMT1-Stealth RNA was designed on line and synthesized by the Invitrogen Company . The Panc-1 cell line was transfected by the stealth RNAi in 50nM concentration of the lipofectamine 2000. The fluorescence of each group was observed under the microscope after 24 h, 48 h and 72 h respectively. The expression of DNMT1 mRNA was detected by RT-PCRs the expression of DNMT1 protein was detected by Western blotting after transfected Stealth RNAi for 48 hours. Results: Forty-eight hours after the Panel cell transfected by stealth RNAi, the efficiency of the transfection was between 50% to 60%. The expression of mRNA and protein of the 3rd RNAi transfected group decreased 67. 8% and 68% respectively comparing with the control (P< 0.05). Conclusions: The RNAi targeting DNMT1 gene is transfected to the Panc-1 cell line, which could down regulated the DNMT1 gene expression both on mRNA and protein. This study provides a new gene therapy approach for pancreatic cancer management in the future.关键词
胰腺癌/RNA干扰/甲基化转移酶1Key words
Pancreatic cancer/ RNA interfering(RNAi)/ DNA methyltransferasel (DNMT1)分类
医药卫生引用本文复制引用
卜淑蕊,钱家鸣..Stealth核糖核苷酸干扰抑制胰腺癌细胞DNMT1表达的研究[J].中国临床医学,2011,18(6):757-760,4.基金项目
博士后科学基金资助项目(编号:2005038379) （编号:2005038379）
