中国临床药学杂志2012，Vol.21Issue(1)：7-12,6.
全反式维甲酸对糖尿病大鼠肾脏组织BMP-7表达及细胞外基质代谢的影响
Effects of all-trans retinoic acid on the expression of bone morphogenetic protein-7 and the metabolism of extreacellular matrix in kidney tissues of streptozotocin-induced diabetic rats
姚春萌 1郭汉城 1刘俊兰 1关天俊 1凌毅生1
作者信息
- 1. 厦门大学附属中山医院暨福建医科大学教学医院肾内科,厦门361004
- 折叠
摘要
Abstract
AIM To explore the effects of all-trans retinoic acid (atRA) on the expression of bone morphogenetic protein-7(BMP-7), transforming growth factor-)β1 (TGF-β1) and the metabolism of collagen IV .collagen 1 in kidney tissues of streptozotocin (STZ) -induced diabetic rats. METHODS Rats injected with STZ were randomly divided into model group (DM)and therapy group (DM + atRA). Other 12 normal rats were used as control group(NC). The treatment was lasted for 8 weeks or 12 weeks, and renal pathological change was evaluated by light and electron microscopy. The expression of BMP-7, TGF-β1 and collagen IV.collagen III in kidney tissues were tested by immunohistochemistry technique. The mRNA expression of BMP-7 and TGF-β1 in kidney tissues was quantitatively determined through real time reverse transcription polymerase chain reaction (real time RT-PCR) . RESULTS The expression of BMP-7 in kidney in DM + atRA group was significantly higher than that in DM group ( P < 0.01), but the expression of TGF-β1 , collagen HI and IV of kidney in DM + atRA group was significantly lower than that in DM group ( P < 0.01). And the levels ofprotein exprssion of BMP-7 were negatively correlated with the levels of TGF-β1 (r= - 0.86, P < 0.01) , collagen IV (r= -0.75, P<0.01)and collagen DI(r= - 0.71, P < 0.01) .respectively. The levels of mRNA exprssion of BMP-7 in kidney in DM + atRA group were higher than those in DM group (P < 0.01) , but the levels of TGF-β1 lower than those in DM group ( P < 0.01). Also the levels of mRNA exprssion of BMP-7 were negatively correlated with the levels of TGF-β1(r = - 0.83, P < 0.01). CONCLUSION The atRA can relieve renal injury and inhibit renal fibrosis in rats with diabetic nephropathy induced by STZ , which might be related to the up-regulating of the expression of BMP-7 and the down-regulating the expression of TGF-f)! Expression in kidney tissues.关键词
全反式维甲酸/糖尿病肾病/细胞外基质/骨形成蛋白-7/转化生长因子-β1Key words
all-trans retinoic acid/ diabetic nephropathy/ extracellular matrix/ bone morphogenetic protein-7/ transforming growth factor-β1引用本文复制引用
姚春萌,郭汉城,刘俊兰,关天俊,凌毅生..全反式维甲酸对糖尿病大鼠肾脏组织BMP-7表达及细胞外基质代谢的影响[J].中国临床药学杂志,2012,21(1):7-12,6.
