中国美容医学2012，Vol.21Issue(3)：406-409,4.
EGFP和CM-Dil示踪骨髓间充质干细胞构建组织工程骨的体内研究
The in vivo study of tissue engineered bone constructed with EGFP and CM-Dil labeled BMSCs
武京国 1谢方南 1马慧雨 1王黔 1曹谊林 1肖苒1
作者信息
- 1. 北京协和医学院·中国医学科学院·整形外科医院研究中心,北京,100144
- 折叠
摘要
Abstract
Objective To observe the effect of BMSCs in fabricating tissue engineering bone in vivo using EGFP andCM-Dil labeling technology. Methods BMSCs isolated from Beagle Dogs were labeled using EGFP and CM -Dilseparately and the proliferation abilities were analyzed by MTT assay. BMSCs were seeded onto the coral scaffolds andcultured in the osteogenic medium for 7 days.Then the BMSCs/Coral constructs were implanted into the nude micessubcutaneously.The constructs were divided into three groups:Unlabeled group,EGFP group,CM -Dil group.Thespecimens were collected respectively at 4,8 and 12 weeks after implantation and tissue engineered bone wasevaluated by HE staining.The seeded BMSCs were traced by immunohistochemistry staining of GFP in the EGFP groupand directly observed in the frozen section under the fluorescence microscope in the CM-Dil. Results BMSCs werelabeled efficiently by both GFP and CM-Dil labeling technology and there was no statistical significance in BMSCsproliferation after labeling (P>0.05).The histological observation of three groups showed that there were engineered boneformed around the pores of the scaffolds after 12 weeks implantation. The labeled BMSCs were decreased graduallyand could be detected up to 12 weeks in vivo. Conclusion EGFP and CM-Dil can be used to label and trace theseeded BMSCs in tissue engineered bone formation in vivo.关键词
EGFP/CM-Dil/骨髓间充质干细胞/示踪/组织工程骨Key words
EGFP/ CM-Dil/ BMSCs/ lable/ tissue engineering bone分类
生物科学引用本文复制引用
武京国,谢方南,马慧雨,王黔,曹谊林,肖苒..EGFP和CM-Dil示踪骨髓间充质干细胞构建组织工程骨的体内研究[J].中国美容医学,2012,21(3):406-409,4.
