中国美容医学2012，Vol.21Issue(4)：576-579,4.
阻断Notch信号抑制角质形成细胞的分泌功能的研究
Blocking Notch signal pathway suppress profibrotic growth factor production in keratinocyte
摘要
Abstract
Objective To study the impact of Notch signaling on the profibrotic growth factor production in keratinocyte. Methods To mimic the components of the acute wound microenvironment, we used serum stimulation model in vitro. Results We found that expression of Notch-1 and Jagged-1 in keratinocytes was significantly higher after serum stimulation, and shown time independent. After keratinocytes serum stimulation, the expression of P21 was significantly up-regulated and had a peak at 6h, while the expression of P63 was down-regulated with the biggest fall at 6h. Besides, the result of real time PCR suggested that nearly all the detected profibrotic growth factors had a peak at 12h after serum stimulation, in absence of DAPT. DAPT decreased the expression of profibrotic growth factor production at all the indicated time, and had the most potent effect at 12h. But in presence and absence DAPT, the variation tendency of TGF-P, expression was nearly the same, so was VEGF. Maybe this is related to the low dose of DAPT(10pM). Conclusion Blocking Notch signaling pathway can suppress probrotic growth factor production in keratinocyte.关键词
瘢痕/Notch信号/角质形成细胞/血清刺激模型/纤维化相关因子Key words
cicatrix/Notch signal pathway/keratinocyte/serum stimulation model/profibrotic growth factor分类
生物科学引用本文复制引用
李冰,刁建升,楚菲菲,王大雷,郭树忠,夏炜..阻断Notch信号抑制角质形成细胞的分泌功能的研究[J].中国美容医学,2012,21(4):576-579,4.基金项目
国家自然科学基金(30371469,30801190),陕西省攻关项目(2009K0167) （30371469,30801190）
