中国美容医学2012，Vol.21Issue(4)：593-595,3.
阿司匹林对正常人表皮黑素细胞系PIG1细胞黑素生成的影响
Effects of aspirin on melanogenesis of human epidermal melanocyte cell line PIG1 cells in vitro
摘要
Abstract
Objective To investigate the effects of aspirin on cell viability, tyrosinase activity and melanogenesis of human epidermal melanocyte cell line PIG1 cells in vitro. Methods After treating PIG1 cell with aspirin in different concentrations for 72h, we employed CCK-8 assay, oxidative DOPA reaction and NaOH method to measure the cell viability, tyrosinase activity and melanogenesis of PIG1 cells respectively. The morphologic changes of PIG1 cells were observed via light microscope. Results Aspirin had no significant effect on the cell viability of PIG1 cells when the concentrations were lower than 500Mmol/L. The melanogenesis of PIG1 cells was remarkably decreased in a concentration-dependent manner by aspirin at the concentrations higher than 125Mmol/L (P<0.05). However the tyrosinase activity had no significant change after aspirin treatment (P>0.05). Conclusion Aspirin can inhibit the melanogenesis of PIG1 cells, thus it may be developed as a clinical treatment of hyperpigmented skin diseases.关键词
阿司匹林/黑素细胞/黑素生成/酪氨酸酶Key words
aspirin/melanocyte/melanogenesis/tyrosinase分类
生物科学引用本文复制引用
汤玲珍,坚哲,刘邦民,李凯,李强,李春英,高天文..阿司匹林对正常人表皮黑素细胞系PIG1细胞黑素生成的影响[J].中国美容医学,2012,21(4):593-595,3.基金项目
国家自然科学基金资助(NO.81130032 and NO.30972642) （NO.81130032 and NO.30972642）
