中国免疫学杂志2012，Vol.28Issue(2)：99-103,5.DOI:10.3969/j.issn.1000-484X.2012.02.001
MAPKs在anti-β2GPI/β2GPI刺激THP-1细胞表达TF过程中的作用探讨
The roles of MAPKs in anti-β2GPI/β2GPI-induced tissue factor expression on THP-1 cells
摘要
Abstract
Objective: To investigate whether mitogen-activated protein kinases ( MAPKs ) are involved in anti-β2GPI/β2GPI-induced tissue factor ( TF ) expression in THP-1 cells. Methods :TF expression in THP-1 cells was measured by Real-time PCR or TF activity after stimulating with anti-β2GPI/β2GPI complex. The activation of p38, ERK1/2, and JNK induced by anti-β2 GPI/β2 GPI complex were determined by Western blot using phosphor-specific antibodies. Inhibitors against p38, ERK1/2, JNK ( SB203580, U0126, SP600125 ) were used to test whether the blockade of these pathway would interrupt the induction of TF expression in THP-1 cells by anti-β2GPI/β2GPI complex. Results:p38,ERKl/2,and JNK were active in a time-dependent manner in THP-1 cells upon treatment with anti-β2GPI/β2GPI complex ( 100 μg/ml). Induction of TF mRNA expression and TF activity by anti-β2GPI/β2GPI complex ( 100 μg/ml ) in THP-1 cells could be blocked by inhibitors against the MAPKs,SB203580 ( 10 μmol/L),U0126 ( 5 μmol/L),SP600125 ( 90 nmol/L). Conclusion:MAPKs can be activated by anti-β2GPI/β2GPI in THP-1 cells and is involved in the induction of TF expression.关键词
抗磷脂综合征/Anti-β2GPI/β2GPI/丝裂原活化蛋白激酶/组织因子Key words
Antiphospholipid syndrome/ Anti-β2 GPI/β2 GPI/ MAPKs/ Tissue factor分类
医药卫生引用本文复制引用
陈东东,周红,解鸿翔,张先梅,夏龙飞,王婷,王海波..MAPKs在anti-β2GPI/β2GPI刺激THP-1细胞表达TF过程中的作用探讨[J].中国免疫学杂志,2012,28(2):99-103,5.基金项目
本文受国家自然科学基金资助项目(No.30971301)和江苏大学学生科研课题项目(No.10A108)资助 （No.30971301）
